UniProt Accession

 NEDD8_HUMAN; Q15843; Q3SXN8; Q6LES6 

Genbank Protein ID

 D23662; CR407662; BC104200; BC104201; BC104664 

Genbank Nucleotide ID

 BAA04889.1; CAG28590.1; AAI04201.1; AAI04202.1; AAI04665.1 

Protein Name

 NEDD8 

Protein Synonyms/Alias

 Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 

Gene Name

 NEDD8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
4	****MLIKVKTLTGK	ubiquitination	[1]
6	**MLIKVKTLTGKEI	ubiquitination	[1, 2, 3, 4]
11	KVKTLTGKEIEIDIE	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
22	IDIEPTDKVERIKER	ubiquitination	[1, 2, 4, 5]
27	TDKVERIKERVEEKE	ubiquitination	[2, 3]
33	IKERVEEKEGIPPQQ	ubiquitination	[1, 2, 3, 4]
48	QRLIYSGKQMNDEKT	acetylation	[10]
48	QRLIYSGKQMNDEKT	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
54	GKQMNDEKTAADYKI	ubiquitination	[1, 2, 3, 4, 6, 7, 9]
60	EKTAADYKILGGSVL	ubiquitination	[1, 2, 3, 4, 7, 11]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail.
 Yamoah K, Oashi T, Sarikas A, Gazdoiu S, Osman R, Pan ZQ.
 Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12230-5. [PMID: 18723677]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [11] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. 

Sequence Annotation

 REGION 70 72 Interaction with UBE1C.
 CROSSLNK 76 76 Glycyl lysine isopeptide (Gly-Lys)  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Isopeptide bond; Nucleus; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 81 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0009653; P:anatomical structure morphogenesis; TAS:ProtInc.
 GO:0008104; P:protein localization; IEA:Compara.
 GO:0045116; P:protein neddylation; IDA:MGI.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0014070; P:response to organic cyclic compound; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:ProtInc. 

Interpro

 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019956; Ubiquitin_subgr.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF00240; ubiquitin 

SMART

 SM00213; UBQ 

PROSITE

 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 

PRINTS

 PR00348; UBIQUITIN.