CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012023
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dual specificity mitogen-activated protein kinase kinase 5 
Protein Synonyms/Alias
 MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5 
Gene Name
 MAP2K5 
Gene Synonyms/Alias
 MEK5; MKK5; PRKMK5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
121ERNIHGLKVNTRAGPubiquitination[1]
146SLPSNSLKKSSAELKubiquitination[1]
154KSSAELKKILANGQMubiquitination[1]
298VNTRGQVKLCDFGVSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. 
Sequence Annotation
 DOMAIN 18 97 OPR.
 DOMAIN 166 409 Protein kinase.
 NP_BIND 172 180 ATP (By similarity).
 REGION 18 25 Interaction with MAPK7 (By similarity).
 REGION 64 68 Interaction with MAP3K2/MAP3K3 (By
 REGION 117 131 Interaction with MAPK7 (By similarity).
 ACT_SITE 283 283 Proton acceptor (By similarity).
 BINDING 195 195 ATP.
 MOD_RES 311 311 Phosphoserine.
 MOD_RES 315 315 Phosphothreonine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 448 AA 
Protein Sequence
MLWLALGPFP AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ VLPEATTTAF 60
EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE PLQIFPRACK PPGERNIHGL 120
KVNTRAGPSQ HSSPAVSDSL PSNSLKKSSA ELKKILANGQ MNEQDIRYRD TLGHGNGGTV 180
YKAYHVPSGK ILAVKVILLD ITLELQKQIM SELEILYKCD SSYIIGFYGA FFVENRISIC 240
TEFMDGGSLD VYRKMPEHVL GRIAVAVVKG LTYLWSLKIL HRDVKPSNML VNTRGQVKLC 300
DFGVSTQLVN SIAKTYVGTN AYMAPERISG EQYGIHSDVW SLGISFMELA LGRFPYPQIQ 360
KNQGSLMPLQ LLQCIVDEDS PVLPVGEFSE PFVHFITQCM RKQPKERPAP EELMGHPFIV 420
QFNDGNAAVV SMWVCRALEE RRSQQGPP 448 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005819; C:spindle; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; TAS:ProtInc.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
 GO:0000187; P:activation of MAPK activity; ISS:BHF-UCL.
 GO:0071499; P:cellular response to laminar fluid shear stress; TAS:BHF-UCL.
 GO:0070375; P:ERK5 cascade; IEA:Compara.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; ISS:BHF-UCL.
 GO:0090051; P:negative regulation of cell migration involved in sprouting angiogenesis; ISS:BHF-UCL.
 GO:2000342; P:negative regulation of chemokine (C-X-C motif) ligand 2 production; ISS:BHF-UCL.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; ISS:BHF-UCL.
 GO:0034115; P:negative regulation of heterotypic cell-cell adhesion; ISS:BHF-UCL.
 GO:0045415; P:negative regulation of interleukin-8 biosynthetic process; ISS:BHF-UCL.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:BHF-UCL.
 GO:0060761; P:negative regulation of response to cytokine stimulus; ISS:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:BHF-UCL.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:BHF-UCL. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000270; OPR_PB1.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00564; PB1
 PF00069; Pkinase 
SMART
 SM00666; PB1
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS