CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011156
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histone-lysine N-methyltransferase, H3 lysine-79 specific 
Protein Synonyms/Alias
 Disrupter of telomere silencing protein 1; Histone H3-K79 methyltransferase; H3-K79-HMTase; Lysine N-methyltransferase 4 
Gene Name
 DOT1 
Gene Synonyms/Alias
 KMT4; PCH1; YDR440W; D9461.26 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
43KGTDMQTKSLSSYSKubiquitination[1]
50KSLSSYSKGTLLSKQubiquitination[1]
99GLVPLVEKVIPPIHKacetylation[2]
336DHLETIDKIPRSFIHacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones. Can bind to DNA (in vitro). 
Sequence Annotation
 DOMAIN 254 568 DOT1.
 REGION 158 172 Required for interaction with nucleosomes
 REGION 372 375 S-adenosyl-L-methionine binding.
 REGION 395 404 S-adenosyl-L-methionine binding.
 REGION 459 460 S-adenosyl-L-methionine binding.
 BINDING 422 422 S-adenosyl-L-methionine.  
Keyword
 3D-structure; Chromatin regulator; Complete proteome; DNA-binding; Methyltransferase; Nucleus; Reference proteome; Repeat; S-adenosyl-L-methionine; Transcription; Transcription regulation; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 582 AA 
Protein Sequence
MGGQESISNN NSDSFIMSSP NLDSQESSIS PIDEKKGTDM QTKSLSSYSK GTLLSKQVQN 60
LLEEANKYDP IYGSSLPRGF LRDRNTKGKD NGLVPLVEKV IPPIHKKTNN RNTRKKSSTT 120
TKKDVKKPKA AKVKGKNGRT NHKHTPISKQ EIDTAREKKP LKKGRANKKN DRDSPSSTFV 180
DWNGPCLRLQ YPLFDIEYLR SHEIYSGTPI QSISLRTNSP QPTSLTSDND TSSVTTAKLQ 240
SILFSNYMEE YKVDFKRSTA IYNPMSEIGK LIEYSCLVFL PSPYAEQLKE TILPDLNASF 300
DNSDTKGFVN AINLYNKMIR EIPRQRIIDH LETIDKIPRS FIHDFLHIVY TRSIHPQANK 360
LKHYKAFSNY VYGELLPNFL SDVYQQCQLK KGDTFMDLGS GVGNCVVQAA LECGCALSFG 420
CEIMDDASDL TILQYEELKK RCKLYGMRLN NVEFSLKKSF VDNNRVAELI PQCDVILVNN 480
FLFDEDLNKK VEKILQTAKV GCKIISLKSL RSLTYQINFY NVENIFNRLK VQRYDLKEDS 540
VSWTHSGGEY YISTVMEDVD ESLFSPAARG RRNRGTPVKY TR 582 
Gene Ontology
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0031151; F:histone methyltransferase activity (H3-K79 specific); IDA:SGD.
 GO:0031493; F:nucleosomal histone binding; IDA:SGD.
 GO:0006348; P:chromatin silencing at telomere; IMP:SGD.
 GO:0044783; P:G1 DNA damage checkpoint; IMP:SGD.
 GO:0070911; P:global genome nucleotide-excision repair; IMP:SGD.
 GO:0031573; P:intra-S DNA damage checkpoint; IMP:SGD.
 GO:0051598; P:meiotic recombination checkpoint; IGI:SGD.
 GO:0006301; P:postreplication repair; IGI:SGD.
 GO:0000725; P:recombinational repair; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR013110; DOT1.
 IPR025789; Histone_H3-K79_MeTrfase.
 IPR021162; Histone_H3-K79_MeTrfase_fungi. 
Pfam
 PF08123; DOT1 
SMART
  
PROSITE
 PS51569; DOT1 
PRINTS