CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017272
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein CASC3 
Protein Synonyms/Alias
 Cancer susceptibility candidate gene 3 protein homolog; Metastatic lymph node gene 51 protein homolog; MLN 51 homolog; Protein barentsz; Btz; mBtz 
Gene Name
 Casc3 
Gene Synonyms/Alias
 Mln51 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
114ENSKVELKSEANDAAubiquitination[1]
174LDDDEDRKNPAYIPRubiquitination[1]
182NPAYIPRKGLFFEHDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA- helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport (By similarity). 
Sequence Annotation
 REGION 134 280 Necessary for RNA-binding, interaction
 REGION 134 280 Sufficient to form the EJC (By
 REGION 374 698 Necessary for localization in cytoplasmic
 MOTIF 201 207 Nuclear localization signal 1
 MOTIF 251 259 Nuclear localization signal 2
 MOTIF 457 466 Nuclear export signal.
 MOD_RES 115 115 Phosphoserine (By similarity).
 MOD_RES 145 145 Phosphoserine.
 MOD_RES 262 262 Phosphoserine (By similarity).
 MOD_RES 263 263 Phosphoserine.
 MOD_RES 360 360 Phosphoserine.
 MOD_RES 370 370 Phosphoserine (By similarity).
 MOD_RES 472 472 Phosphoserine (By similarity).  
Keyword
 ADP-ribosylation; Coiled coil; Complete proteome; Cytoplasm; mRNA processing; mRNA splicing; mRNA transport; Nonsense-mediated mRNA decay; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Stress response; Translation regulation; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 698 AA 
Protein Sequence
MADRRRQRAS QDTEDEESGA SGSDSGSPAR GGGSCSGSVG GGGSGSLPSQ RGGRGGGLHL 60
RRVESGGAKS AEESECESED GMEGDAVLSD YESAEDSEGE EDYSEEENSK VELKSEANDA 120
ADSSAKEKGE EKPESKGTVT GERQSGDGQE STEPVENKVG KKGPKHLDDD EDRKNPAYIP 180
RKGLFFEHDL RGQTQEEEVR PKGRQRKLWK DEGRWEHDKF REDEQAPKSR QELIALYGYD 240
IRSAHNPDDI KPRRIRKPRF GSSPQRDPNW IGDRSSKSHR HQGPGGNLPP RTFINRNTAG 300
TGRMSASRNY SRSGGFKDGR TSFRPVEVAG QHGGRSAETL KHEASYRSRR LEQTPVRDPS 360
PEPDAPLLGS PEKEEVASET PAAVPDITPP APDRPIEKKS YSRARRTRTK VGDAVKAAEE 420
VPPPSEGLAS TATVPETTPA AKTGNWEAPV DSTTGGLEQD VAQLNIAEQS WSPSQPSFLQ 480
PRELRGVPNH IHMGAGPPPQ FNRMEEMGVQ SGRAKRYSSQ RQRPVPEPPA PPVHISIMEG 540
HYYDPLQFQG PIYTHGDSPA PLPPQGMIVQ PEMHLPHPGL HPHQSPGPLP NPGLYPPPVS 600
MSPGQPPPQQ LLAPTYFSAP GVMNFGNPNY PYAPGALPPP PPPHLYPNTQ APPQVYGGVT 660
YYNPAQQQVQ PKPSPPRRTP QPVSIKPPPP EVVSRGSS 698 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0035145; C:exon-exon junction complex; IEA:Compara.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030529; C:ribonucleoprotein complex; IDA:MGI.
 GO:0042802; F:identical protein binding; ISO:MGI.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0008298; P:intracellular mRNA localization; IDA:MGI.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR018545; Btz_dom. 
Pfam
 PF09405; Btz 
SMART
 SM01044; Btz 
PROSITE
  
PRINTS