UniProt Accession

 METK1_YEAST; P10659; D6VYI4 

Genbank Protein ID

 J03477; U17246; BK006945 

Genbank Nucleotide ID

 AAA66932.1; AAB67461.1; DAA09500.1 

Protein Name

 S-adenosylmethionine synthase 1 

Protein Synonyms/Alias

 AdoMet synthase 1; Methionine adenosyltransferase 1; MAT 1 

Gene Name

 SAM1 

Gene Synonyms/Alias

 ETH10; YLR180W; L9470.9 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
48	VACETAAKTGMIMVF	ubiquitination	[1]
68	KAQLDYQKIVRDTIK	acetylation	[2]
68	KAQLDYQKIVRDTIK	ubiquitination	[1]
76	IVRDTIKKIGYDDSA	ubiquitination	[1]
84	IGYDDSAKGFDYKTC	acetylation	[2]
84	IGYDDSAKGFDYKTC	ubiquitination	[1, 3]
176	TQVTVEYKDDHGRWV	acetylation	[2]
210	EDLRAQLKSEIIEKV	acetylation	[2]
210	EDLRAQLKSEIIEKV	ubiquitination	[1, 3]
216	LKSEIIEKVIPRDML	acetylation	[2]
228	DMLDENTKYFIQPSG	ubiquitination	[1]
272	GGGAFSGKDYSKVDR	ubiquitination	[1]
290	YAARWVAKSLVAAGL	acetylation	[2]
290	YAARWVAKSLVAAGL	ubiquitination	[1]
299	LVAAGLCKRVQVQFS	ubiquitination	[1]
338	EIIDIISKNFDLRPG	acetylation	[2]
338	EIIDIISKNFDLRPG	ubiquitination	[1]
349	LRPGVLVKELDLARP	acetylation	[2]
349	LRPGVLVKELDLARP	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149] 

Functional Description

 Catalyzes the formation of S-adenosylmethionine from methionine and ATP. 

Sequence Annotation

 NP_BIND 118 123 ATP (Potential).
 METAL 18 18 Magnesium (By similarity).
 METAL 44 44 Potassium (By similarity).
 METAL 270 270 Potassium (By similarity).
 METAL 278 278 Magnesium (By similarity).
 BINDING 146 146 ATP (Potential).  

Keyword

 ATP-binding; Cobalt; Complete proteome; Direct protein sequencing; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 382 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004478; F:methionine adenosyltransferase activity; IGI:SGD.
 GO:0006555; P:methionine metabolic process; IMP:SGD.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0006556; P:S-adenosylmethionine biosynthetic process; IMP:SGD. 

Interpro

 IPR022631; ADOMET_SYNTHASE_CS.
 IPR022630; S-AdoMet_synt_C.
 IPR022629; S-AdoMet_synt_central.
 IPR022628; S-AdoMet_synt_N.
 IPR002133; S-AdoMet_synthetase.
 IPR022636; S-AdoMet_synthetase_sfam. 

Pfam

 PF02773; S-AdoMet_synt_C
 PF02772; S-AdoMet_synt_M
 PF00438; S-AdoMet_synt_N 

SMART

  

PROSITE

 PS00376; ADOMET_SYNTHASE_1
 PS00377; ADOMET_SYNTHASE_2 

PRINTS