CPLM-005308

Genbank Nucleotide ID

Growth arrest and DNA damage-inducible protein GADD45 alpha

Protein Synonyms/Alias

DNA damage-inducible transcript 1 protein; DDIT-1

Homo sapiens (Human)

Position	Peptide	Type	References
12	EFSAGEQKTERMDKV	ubiquitination	[1, 2]
18	QKTERMDKVGDALEE	ubiquitination	[1, 3, 4]
29	ALEEVLSKALSQRTI	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
Xu G, Paige JS, Jaffrey SR.
Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[7] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.

REGION 1 16 Intrinsically disordered.
REGION 105 118 Intrinsically disordered.

3D-structure; Alternative splicing; Cell cycle; Complete proteome; DNA damage; Growth arrest; Nucleus; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005634; C:nucleus; IDA:LIFEdb.
GO:0006915; P:apoptotic process; TAS:ProtInc.
GO:0007050; P:cell cycle arrest; TAS:ProtInc.
GO:0071479; P:cellular response to ionizing radiation; IMP:BHF-UCL.
GO:0071260; P:cellular response to mechanical stimulus; IEP:UniProtKB.
GO:0007098; P:centrosome cycle; IEA:Compara.
GO:0006281; P:DNA repair; TAS:ProtInc.
GO:0006469; P:negative regulation of protein kinase activity; IEA:Compara.
GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IMP:BHF-UCL.
GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; TAS:ProtInc.
GO:0042770; P:signal transduction in response to DNA damage; IMP:BHF-UCL.

IPR024824; GADD45.
IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.

PF01248; Ribosomal_L7Ae