CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013314
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 10B 
Protein Synonyms/Alias
 Rab27A-GAP-beta 
Gene Name
 TBC1D10B 
Gene Synonyms/Alias
 FP2461 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
368IPSSLRAKAWQYLSNubiquitination[1, 2, 3]
398ERAPGDPKWLDVIEKubiquitination[1, 3]
405KWLDVIEKDLHRQFPubiquitination[1, 2, 3]
433QDLYRILKAYTIYRPacetylation[4]
666LLSLPGLKSRGSRAAubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 Acts as GTPase-activating protein for RAB3A, RAB22A, RAB27A, AND RAB35. Does not act on RAB2A and RAB6A. 
Sequence Annotation
 DOMAIN 360 548 Rab-GAP TBC.
 MOD_RES 658 658 Phosphoserine.
 MOD_RES 661 661 Phosphoserine.
 MOD_RES 678 678 Phosphoserine.
 MOD_RES 687 687 Phosphoserine.
 MOD_RES 707 707 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; GTPase activation; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 808 AA 
Protein Sequence
METGTAPLVA PPRRHGAPAA PSPPPRGSRA GPVVVVAPGP PVTTATSAPV TLVAPGEARP 60
AWVPGSAETS APAPAPAPAP APAVTGSTVV VLTLEASPEA PKPQLPSGPE SPEPAAVAGV 120
ETSRALAAGA DSPKTEEARP SPAPGPGTPT GTPTRTPSRT APGALTAKPP LAPKPGTTVA 180
SGVTARSASG QVTGGHGAAA ATSASAGQAP EDPSGPGTGP SGTCEAPVAV VTVTPAPEPA 240
ENSQDLGSTS SLGPGISGPR GQAPDTLSYL DSVSLMSGTL ESLADDVSSM GSDSEINGLA 300
LRKTDKYGFL GGSQYSGSLE SSIPVDVARQ RELKWLDMFS NWDKWLSRRF QKVKLRCRKG 360
IPSSLRAKAW QYLSNSKELL EQNPGKFEEL ERAPGDPKWL DVIEKDLHRQ FPFHEMFAAR 420
GGHGQQDLYR ILKAYTIYRP DEGYCQAQAP VAAVLLMHMP AEQAFWCLVQ ICDKYLPGYY 480
SAGLEAIQLD GEIFFALLRR ASPLAHRHLR RQRIDPVLYM TEWFMCIFAR TLPWASVLRV 540
WDMFFCEGVK IIFRVALVLL RHTLGSVEKL RSCQGMYETM EQLRNLPQQC MQEDFLVHEV 600
TNLPVTEALI ERENAAQLKK WRETRGELQY RPSRRLHGSR AIHEERRRQQ PPLGPSSSLL 660
SLPGLKSRGS RAAGGAPSPP PPVRRASAGP APGPVVTAEG LHPSLPSPTG NSTPLGSSKE 720
TRKQEKERQK QEKERQKQEK EREKERQKQE KEREKQEKER EKQEKERQKQ EKKAQGRKLS 780
LRRKADGPPG PHDGGDRPSA EARQDAYF 808 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005097; F:Rab GTPase activator activity; IDA:UniProtKB. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS