CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004815
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Viral transcription factor IE2 
Protein Synonyms/Alias
 IE2; Protein UL122 
Gene Name
 UL122 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) 
NCBI Taxa ID
 10360 
Lysine Modification
Position
Peptide
Type
References
175PIMLPLIKQEDIKPEsumoylation[1, 2, 3, 4]
180LIKQEDIKPEPDFTIsumoylation[1, 2, 3, 4]
Reference
 [1] Covalent modification of the transactivator protein IE2-p86 of human cytomegalovirus by conjugation to the ubiquitin-homologous proteins SUMO-1 and hSMT3b.
 Hofmann H, Flöss S, Stamminger T.
 J Virol. 2000 Mar;74(6):2510-24. [PMID: 10684265]
 [2] Evaluation of interactions of human cytomegalovirus immediate-early IE2 regulatory protein with small ubiquitin-like modifiers and their conjugation enzyme Ubc9.
 Ahn JH, Xu Y, Jang WJ, Matunis MJ, Hayward GS.
 J Virol. 2001 Apr;75(8):3859-72. [PMID: 11264375]
 [3] Sumoylation of topoisomerase I is involved in its partitioning between nucleoli and nucleoplasm and its clearing from nucleoli in response to camptothecin.
 Rallabhandi P, Hashimoto K, Mo YY, Beck WT, Moitra PK, D'Arpa P.
 J Biol Chem. 2002 Oct 18;277(42):40020-6. [PMID: 12149243]
 [4] Importance of covalent and noncovalent SUMO interactions with the major human cytomegalovirus transactivator IE2p86 for viral infection.
 Berndt A, Hofmann-Winkler H, Tavalai N, Hahn G, Stamminger T.
 J Virol. 2009 Dec;83(24):12881-94. [PMID: 19812159] 
Functional Description
 Stimulates viral early and late gene expression and thus play a crucial role in the regulation of productive infection. In addition, activates quiescent cells to re-enter the cell cycle and upregulates several E2F-responsive genes, which are responsible for pushing the cell into S phase. In S-phase, inhibits cellular DNA synthesis and blocks further cell cycle progression. 
Sequence Annotation
 REGION 200 208 Non-covalent SUMO1 binding region (SIM).
 CROSSLNK 175 175 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 180 180 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Activator; Complete proteome; DNA-binding; Early protein; G0/G1 host cell cycle checkpoint dysregulation by virus; G1/S host cell cycle checkpoint dysregulation by virus; Host nucleus; Host-virus interaction; Isopeptide bond; Metal-binding; Modulation of host cell cycle by virus; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 580 AA 
Protein Sequence
MESSAKRKMD PDNPDEGPSS KVPRPETPVT KATTFLQTML RKEVNSQLSL GDPLFPELAE 60
ESLKTFEQVT EDCNENPEKD VLAELGDILA QAVNHAGIDS SSTGPTLTTH SCSVSSAPLN 120
KPTPTSVAVT NTPLPGASAT PELSPRKKPR KTTRPFKVII KPPVPPAPIM LPLIKQEDIK 180
PEPDFTIQYR NKIIDTAGCI VISDSEEEQG EEVETRGATA SSPSTGSGTP RVTSPTHPLS 240
QMNHPPLPDP LGRPDEDSSS SSSSSCSSAS DSESESEEMK CSSGGGASVT SSHHGRGGFG 300
GAASSSLLSC GHQSSGGAST GPRKKKSKRI SELDNEKVRN IMKDKNTPFC TPNVQTRRGR 360
VKIDEVSRMF RNTNRSLEYK NLPFTIPSMH QVLDEAIKAC KTMQVNNKGI QIIYTRNHEV 420
KSEVDAVRCR LGTMCNLALS TPFLMEHTMP VTHPPEVAQR TADACNEGVK AAWSLKELHT 480
HQLCPRSSDY RNMIIHAATP VDLLGALNLC LPLMQKFPKQ VMVRIFSTNQ GGFMLPIYET 540
AAKAYAVGQF EQPTETPPED LDTLSLAIEA AIQDLRNKSQ 580 
Gene Ontology
 GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
 GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0050792; P:regulation of viral reproduction; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR010855; Cytomega_IE1.
 IPR005028; Herpes_IE2_3. 
Pfam
 PF07340; Herpes_IE1
 PF03361; Herpes_IE2_3 
SMART
  
PROSITE
  
PRINTS