CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004038
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial 
Protein Synonyms/Alias
 Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Pyruvate dehydrogenase complex component E2; PDC-E2; PDCE2 
Gene Name
 LAT1 
Gene Synonyms/Alias
 ODP2; PDA2; YNL071W; N2374 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
98ILVPEGTKDIPVNKPacetylation[1]
104TKDIPVNKPIAVYVEacetylation[1]
113IAVYVEDKADVPAFKacetylation[1]
120KADVPAFKDFKLEDSacetylation[1]
123VPAFKDFKLEDSGSDacetylation[1]
156EAPAEETKTSAPEAKacetylation[1]
181IFASPLAKTIALEKGacetylation[1]
187AKTIALEKGISLKDVacetylation[1]
192LEKGISLKDVHGTGPacetylation[1]
205GPRGRITKADIESYLacetylation[1]
214DIESYLEKSSKQSSQacetylation[1]
287SSKISISKLLKLRQSacetylation[1]
302LNATANDKYKLSINDacetylation[1]
304ATANDKYKLSINDLLacetylation[1]
365IVKNCEAKGLSQISNubiquitination[2]
375SQISNEIKELVKRARacetylation[1]
462HRTIDGAKGAEFMKEacetylation[1]
468AKGAEFMKELKTVIEacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). 
Sequence Annotation
 DOMAIN 35 109 Lipoyl-binding.
 ACT_SITE 455 455 Potential.
 ACT_SITE 459 459 Potential.
 MOD_RES 75 75 N6-lipoyllysine.  
Keyword
 Acyltransferase; Complete proteome; Direct protein sequencing; Lipoyl; Mitochondrion; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 482 AA 
Protein Sequence
MSAFVRVVPR ISRSSVLTRS LRLQLRCYAS YPEHTIIGMP ALSPTMTQGN LAAWTKKEGD 60
QLSPGEVIAE IETDKAQMDF EFQEDGYLAK ILVPEGTKDI PVNKPIAVYV EDKADVPAFK 120
DFKLEDSGSD SKTSTKAQPA EPQAEKKQEA PAEETKTSAP EAKKSDVAAP QGRIFASPLA 180
KTIALEKGIS LKDVHGTGPR GRITKADIES YLEKSSKQSS QTSGAAAATP AAATSSTTAG 240
SAPSPSSTAS YEDVPISTMR SIIGERLLQS TQGIPSYIVS SKISISKLLK LRQSLNATAN 300
DKYKLSINDL LVKAITVAAK RVPDANAYWL PNENVIRKFK NVDVSVAVAT PTGLLTPIVK 360
NCEAKGLSQI SNEIKELVKR ARINKLAPEE FQGGTICISN MGMNNAVNMF TSIINPPQST 420
ILAIATVERV AVEDAAAENG FSFDNQVTIT GTFDHRTIDG AKGAEFMKEL KTVIENPLEM 480
LL 482 
Gene Ontology
 GO:0005967; C:mitochondrial pyruvate dehydrogenase complex; IDA:SGD.
 GO:0004742; F:dihydrolipoyllysine-residue acetyltransferase activity; IDA:SGD.
 GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:SGD. 
Interpro
 IPR003016; 2-oxoA_DH_lipoyl-BS.
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR004167; E3-bd.
 IPR006257; LAT1.
 IPR027189; LAT1_fungal.
 IPR011053; Single_hybrid_motif. 
Pfam
 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl
 PF02817; E3_binding 
SMART
  
PROSITE
 PS50968; BIOTINYL_LIPOYL
 PS00189; LIPOYL 
PRINTS