CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008184
UniProt Accession
Genbank Protein ID
 D26090 
Genbank Nucleotide ID
Protein Name
 DNA replication licensing factor MCM5 
Protein Synonyms/Alias
 CDC46 homolog; P1-CDC46 
Gene Name
 Mcm5 
Gene Synonyms/Alias
 Cdc46; Mcmd5 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
272MGIYSIKKFGLNPSKacetylation[1]
349IFGGMDMKKAIACLLubiquitination[2]
395QLLKFVEKCSPIGVYacetylation[1, 3]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). 
Sequence Annotation
 DOMAIN 330 536 MCM.
 NP_BIND 380 387 ATP (Potential).
 MOTIF 511 514 Arginine finger.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 391 391 N6-acetyllysine (By similarity).
 MOD_RES 395 395 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; ATP-binding; Cell cycle; Complete proteome; DNA replication; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 733 AA 
Protein Sequence
MSGFDDPGIF YSDSFGGDPG AEEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK 60
RHYNLGEYWI EVEMEDLASF DEELADHLHK QPAEHLQLLE EAAKEVADEV TRPRPAGDEL 120
LQDIQVMLKS DASPSSIRIL KSDMMSHLVK IPGIIISASA VRAKATRISI QCRSCHNTLT 180
NIAMPRPRGL CLPRKCNMDQ AGRPKCPLDP YFIMPDKCKC VDFQTLKLQE LPDAVPHGEM 240
PRHMQLYCDR YLCDKVVPGN RVTIMGIYSI KKFGLNPSKG RDRVGVGIRS SYIRVLGIQV 300
DTDGSGRSFA GSVSPQEEEE FRRLAALPNI YELISKSISP SIFGGMDMKK AIACLLFGGS 360
RKRLPDGLTR RGDINLLMLG DPGTAKSQLL KFVEKCSPIG VYTSGKGSSA AGLTASVIRD 420
PSSRNFIMEG GAMVLADGGV VCIDEFDKMR EDDRVAIHEA MEQQTISIAK AGITTTLNSR 480
CSVLAAANSV FGRWDETKGE DNIDFMPTIL SRFDMIFIVK DEHNEERDMM LAKHVMTLHV 540
SALTQTQAVE GEIDLAKMKK FIAYCRARCG PRLSAEAAEK LKNRYIIMRS GARQHERDSD 600
RRSSIPITVR QLEAIVRIAE ALSKMKLQPF ATEADVEEAL RLFQVSTLDA ALSGNLSGVE 660
GFTTQEDQEM LSRIEKQLKR RFAIGSQVSE HSIVQDFTKQ KYPEHAIRKV LQLMLRRGEI 720
QHRMQRKVLY RLK 733 
Gene Ontology
 GO:0042555; C:MCM complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003678; F:DNA helicase activity; IEA:InterPro.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0032508; P:DNA duplex unwinding; IEA:GOC.
 GO:0006270; P:DNA replication initiation; IEA:InterPro. 
Interpro
 IPR008048; MCM5.
 IPR018525; MCM_CS.
 IPR001208; MCM_DNA-dep_ATPase.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00493; MCM 
SMART
 SM00350; MCM 
PROSITE
 PS00847; MCM_1
 PS50051; MCM_2 
PRINTS
 PR01657; MCMFAMILY.
 PR01661; MCMPROTEIN5.