CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010915
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myocyte-specific enhancer factor 2A 
Protein Synonyms/Alias
 Serum response factor-like protein 1 
Gene Name
 MEF2A 
Gene Synonyms/Alias
 MEF2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
68DMDKVLLKYTEYNEPubiquitination[1]
249TGANSLGKVMPTKSPacetylation[2]
403TNQNISIKSEPISPPacetylation[3]
403TNQNISIKSEPISPPsumoylation[3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A calcium-regulated MEF2 sumoylation switch controls postsynaptic differentiation.
 Shalizi A, Gaudillière B, Yuan Z, Stegmüller J, Shirogane T, Ge Q, Tan Y, Schulman B, Harper JW, Bonni A.
 Science. 2006 Feb 17;311(5763):1012-7. [PMID: 16484498]
 [4] PIASx is a MEF2 SUMO E3 ligase that promotes postsynaptic dendritic morphogenesis.
 Shalizi A, Bilimoria PM, Stegmüller J, Gaudillière B, Yang Y, Shuai K, Bonni A.
 J Neurosci. 2007 Sep 12;27(37):10037-46. [PMID: 17855618] 
Functional Description
 Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle- specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. 
Sequence Annotation
 DOMAIN 3 57 MADS-box.
 DNA_BIND 58 86 Mef2-type (Potential).
 REGION 266 283 Required for interaction with MAPKs.
 REGION 289 296 Beta domain.
 MOD_RES 59 59 Phosphoserine; by CK2 (By similarity).
 MOD_RES 98 98 Phosphoserine.
 MOD_RES 235 235 Phosphoserine.
 MOD_RES 249 249 N6-acetyllysine.
 MOD_RES 255 255 Phosphoserine; by MAPK14.
 MOD_RES 312 312 Phosphothreonine; by MAPK7 and MAPK14;
 MOD_RES 312 312 Phosphothreonine; by NLK; alternate.
 MOD_RES 319 319 Phosphothreonine; by MAPK7 and MAPK14.
 MOD_RES 355 355 Phosphoserine; by MAPK7.
 MOD_RES 403 403 N6-acetyllysine; alternate (By
 MOD_RES 408 408 Phosphoserine; by CDK5.
 MOD_RES 453 453 Phosphoserine; by MAPK.
 CROSSLNK 403 403 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Apoptosis; Complete proteome; Developmental protein; Differentiation; Disease mutation; DNA-binding; Isopeptide bond; Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 507 AA 
Protein Sequence
MGRKKIQITR IMDERNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS SNKLFQYAST 60
DMDKVLLKYT EYNEPHESRT NSDIVEALNK KEHRGCDSPD PDTSYVLTPH TEEKYKKINE 120
EFDNMMRNHK IAPGLPPQNF SMSVTVPVTS PNALSYTNPG SSLVSPSLAA SSTLTDSSML 180
SPPQTTLHRN VSPGAPQRPP STGNAGGMLS TTDLTVPNGA GSSPVGNGFV NSRASPNLIG 240
ATGANSLGKV MPTKSPPPPG GGNLGMNSRK PDLRVVIPPS SKGMMPPLSE EEELELNTQR 300
ISSSQATQPL ATPVVSVTTP SLPPQGLVYS AMPTAYNTDY SLTSADLSAL QGFNSPGMLS 360
LGQVSAWQQH HLGQAALSSL VAGGQLSQGS NLSINTNQNI SIKSEPISPP RDRMTPSGFQ 420
QQQQQQQQQQ PPPPPQPQPQ PPQPQPRQEM GRSPVDSLSS SSSSYDGSDR EDPRGDFHSP 480
IVLGRPPNTE DRESPSVKRM RMDAWVT 507 
Gene Ontology
 GO:0000790; C:nuclear chromatin; ISS:BHF-UCL.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:UniProtKB.
 GO:0001105; F:RNA polymerase II transcription coactivator activity; IDA:BHF-UCL.
 GO:0000981; F:sequence-specific DNA binding RNA polymerase II transcription factor activity; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0061337; P:cardiac conduction; ISS:UniProtKB.
 GO:0071277; P:cellular response to calcium ion; IDA:UniProtKB.
 GO:0048813; P:dendrite morphogenesis; ISS:UniProtKB.
 GO:0070375; P:ERK5 cascade; IMP:UniProtKB.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0000002; P:mitochondrial genome maintenance; ISS:UniProtKB.
 GO:0048311; P:mitochondrion distribution; ISS:UniProtKB.
 GO:0007517; P:muscle organ development; NAS:UniProtKB.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0051149; P:positive regulation of muscle cell differentiation; TAS:Reactome.
 GO:0051403; P:stress-activated MAPK cascade; TAS:Reactome.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0055005; P:ventricular cardiac myofibril development; ISS:UniProtKB. 
Interpro
 IPR022102; HJURP_C.
 IPR002100; TF_MADSbox. 
Pfam
 PF12347; HJURP_C
 PF00319; SRF-TF 
SMART
 SM00432; MADS 
PROSITE
 PS00350; MADS_BOX_1
 PS50066; MADS_BOX_2 
PRINTS
 PR00404; MADSDOMAIN.