UniProt Accession

 CARF_HUMAN; Q9NXV6; Q8TBM5; Q9NYH0 

Genbank Protein ID

 AF246705; AK000043; CH471056; BC022270 

Genbank Nucleotide ID

 AAF68967.1; BAA90902.1; EAX04691.1; AAH22270.1 

Protein Name

 CDKN2A-interacting protein 

Protein Synonyms/Alias

 Collaborator of ARF 

Gene Name

 CDKN2AIP 

Gene Synonyms/Alias

 CARF 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
95	LGCRYPQKVMDKILS	ubiquitination	[1]
99	YPQKVMDKILSMAEG	ubiquitination	[2, 3]
423	QSSESSVKFSCKLTN	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Activates p53/TP53 by CDKN2A-dependent and CDKN2A- independent pathways. 

Sequence Annotation

 DOMAIN 462 537 DRBM.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 131 131 Phosphoserine.
 MOD_RES 346 346 Phosphothreonine.  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 580 AA 

Protein Sequence

Gene Ontology

 GO:0001652; C:granular component; IDA:BHF-UCL.
 GO:0005654; C:nucleoplasm; IDA:BHF-UCL.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0002039; F:p53 binding; IDA:BHF-UCL.
 GO:0030308; P:negative regulation of cell growth; IMP:BHF-UCL.
 GO:0009967; P:positive regulation of signal transduction; IMP:BHF-UCL.
 GO:0031647; P:regulation of protein stability; IMP:BHF-UCL. 

Interpro

 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR021859; DUF3469. 

Pfam

 PF11952; DUF3469 

SMART

  

PROSITE

 PS50137; DS_RBD 

PRINTS