UniProt Accession

 CWC2_YEAST; Q12046; D6VRE5 

Genbank Protein ID

 X99000; Z74257; AY693022; BK006938 

Genbank Nucleotide ID

 CAA67482.1; CAA98787.1; AAT93041.1; DAA11655.1 

Protein Name

 Pre-mRNA-splicing factor CWC2 

Protein Synonyms/Alias

 Complexed with CEF1 protein 2; PRP19-associated complex protein 40; Synthetic lethal with CLF1 protein 3 

Gene Name

 CWC2 

Gene Synonyms/Alias

 NTC40; SLC3; YDL209C; D1041 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
152	ALNSKHLKPAQIESR	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre- mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome. 

Sequence Annotation

 DOMAIN 135 228 RRM.
 ZN_FING 67 94 C3H1-type.
 MOD_RES 335 335 Phosphoserine.
 MOD_RES 336 336 Phosphoserine.  

Keyword

 3D-structure; Cell cycle; Complete proteome; Metal-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 339 AA 

Protein Sequence

Gene Ontology

 GO:0000974; C:Prp19 complex; IPI:SGD.
 GO:0071006; C:U2-type catalytic step 1 spliceosome; IDA:SGD.
 GO:0071007; C:U2-type catalytic step 2 spliceosome; IDA:SGD.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0036002; F:pre-mRNA binding; IDA:UniProtKB.
 GO:0017070; F:U6 snRNA binding; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0045292; P:mRNA cis splicing, via spliceosome; IDA:UniProtKB.
 GO:0045787; P:positive regulation of cell cycle; IMP:UniProtKB.
 GO:0033120; P:positive regulation of RNA splicing; IDA:UniProtKB.
 GO:0000387; P:spliceosomal snRNP assembly; IDA:UniProtKB. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR000571; Znf_CCCH. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM
 PS50103; ZF_C3H1 

PRINTS