UniProt Accession

 RAD9A_HUMAN; Q99638; B2RCZ8; Q6FI29; Q96C41 

Genbank Protein ID

 U53174; CR536508; AY766122; AK315348; CH471076; BC014848 

Genbank Nucleotide ID

 AAB39928.1; CAG38746.1; AAU89725.1; BAG37745.1; EAW74605.1; AAH14848.1 

Protein Name

 Cell cycle checkpoint control protein RAD9A 

Protein Synonyms/Alias

 hRAD9; DNA repair exonuclease rad9 homolog A 

Gene Name

 RAD9A 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
15	GNVKVLGKAVHSLSR	ubiquitination	[1]
96	MLEKTVEKCCISLNG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex. 

Sequence Annotation

 REGION 51 91 Possesses 3'-5' exonuclease activity.
 REGION 266 391 Sufficient for interaction with ABL1.
 MOD_RES 28 28 Phosphotyrosine.
 MOD_RES 272 272 Phosphoserine.
 MOD_RES 277 277 Phosphoserine.
 MOD_RES 328 328 Phosphoserine.
 MOD_RES 341 341 Phosphoserine.
 MOD_RES 375 375 Phosphoserine.
 MOD_RES 380 380 Phosphoserine.
 MOD_RES 387 387 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Exonuclease; Hydrolase; Nuclease; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 391 AA 

Protein Sequence

Gene Ontology

 GO:0030896; C:checkpoint clamp complex; IEA:InterPro.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0008408; F:3'-5' exonuclease activity; IDA:UniProtKB.
 GO:0008853; F:exodeoxyribonuclease III activity; IEA:EC.
 GO:0071479; P:cellular response to ionizing radiation; IDA:UniProtKB.
 GO:0000077; P:DNA damage checkpoint; IMP:UniProtKB.
 GO:0006281; P:DNA repair; TAS:ProtInc.
 GO:0006260; P:DNA replication; TAS:Reactome.
 GO:0000076; P:DNA replication checkpoint; TAS:ProtInc.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara. 

Interpro

 IPR026584; Rad9.
 IPR007268; Rad9/Ddc1. 

Pfam

 PF04139; Rad9 

SMART

  

PROSITE

  

PRINTS