CPLM-016313

Genbank Nucleotide ID

Centrosomal protein of 57 kDa

Protein Synonyms/Alias

Cep57; FGF2-interacting protein; Testis-specific protein 57; Translokin

Homo sapiens (Human)

Position	Peptide	Type	References
43	YVVYPSDKPFLNSDL	ubiquitination	[1]
56	DLRRSPSKPTLAYPE	ubiquitination	[1]
74	RAIFSALKNLQDKIR	ubiquitination	[1]
79	ALKNLQDKIRRLELE	ubiquitination	[1]
95	IQAEESVKTLSRETI	ubiquitination	[1, 2, 3, 4, 5]
106	RETIEYKKVLDEQIQ	ubiquitination	[1]
119	IQERENSKNEESKHN	ubiquitination	[1]
124	NSKNEESKHNQELTS	ubiquitination	[1]
145	NKCNLLEKQLEYMRN	ubiquitination	[1]
155	EYMRNMIKHAEMERT	ubiquitination	[1]
167	ERTSVLEKQVSLERE	ubiquitination	[1, 2, 4, 5, 6]
198	LLEQEYNKLTTMQAL	ubiquitination	[1]
209	MQALAEKKMQELEAK	ubiquitination	[1]
230	ERKRMQAKAAELQTG	ubiquitination	[1]
248	NRLIFEDKATPCVPN	ubiquitination	[1, 3]
334	INSIPLAKQVSSRGG	ubiquitination	[1]
396	PTVELKDKLECELEA	ubiquitination	[1]
411	LVGRMEAKANQITKV	ubiquitination	[1]
417	AKANQITKVRKYQAQ	ubiquitination	[1]
462	GITGTTNKKDFMKLR	ubiquitination	[1]
463	ITGTTNKKDFMKLRP	ubiquitination	[1]
476	RPGEKRRKNLQLLKD	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring- like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2, but that of FGF1.

REGION 58 239 centrosome localization domain (CLD) (By
REGION 277 491 Mediates interaction with microtubules

Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Nucleus; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005813; C:centrosome; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
GO:0005874; C:microtubule; IDA:UniProtKB.
GO:0005634; C:nucleus; ISS:HGNC.
GO:0008543; P:fibroblast growth factor receptor signaling pathway; IPI:UniProtKB.
GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
GO:0034453; P:microtubule anchoring; IEA:InterPro.
GO:0051260; P:protein homooligomerization; IEA:Compara.
GO:0000060; P:protein import into nucleus, translocation; ISS:UniProtKB.
GO:0007286; P:spermatid development; ISS:HGNC.

IPR010597; Centrosomal_protein_57kDa.
IPR025913; Cep57_CLD.
IPR024957; Cep57_MT-bd_dom.

PF14073; Cep57_CLD
PF06657; Cep57_MT_bd