CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006501
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycogen debranching enzyme 
Protein Synonyms/Alias
 Glycogen debrancher; 4-alpha-glucanotransferase; Oligo-1,4-1,4-glucantransferase; Amylo-alpha-1,6-glucosidase; Amylo-1,6-glucosidase; Dextrin 6-alpha-D-glucosidase 
Gene Name
 AGL 
Gene Synonyms/Alias
 GDE 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17LLLNEMEKLEKTLFRubiquitination[1]
151ESRLRVAKESGYNMIubiquitination[1]
191DFSRPNRKYTWNDVGubiquitination[1]
265SCDVAEGKYKEKGIPubiquitination[1]
326QENRRVTKSDPNQHLubiquitination[2]
415RLAGHGPKLGPVTRKubiquitination[1]
492ICWGDSVKLRYGNKPubiquitination[1, 3, 4, 5]
679SEERFYTKWNPEALPubiquitination[2, 6, 7]
707AARCAISKLHQELGAubiquitination[1]
771PQMCIPGKIEEVVLEubiquitination[1]
816HIQLNESKIVKQAGVubiquitination[1]
819LNESKIVKQAGVATKubiquitination[1, 5]
875TQFSPHFKSGSLAVDubiquitination[1, 3, 4, 6]
889DNADPILKIPFASLAubiquitination[1, 5, 6, 7]
1075YRLNEITKEKEQCCVubiquitination[2]
1077LNEITKEKEQCCVSLubiquitination[8]
1262NCGTWMDKMGESDRAubiquitination[1]
1291VEIVGLSKSAVRWLLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6- glucosidase in glycogen degradation. 
Sequence Annotation
 REGION 1 ? 4-alpha-glucanotransferase.
 REGION ? 1532 Amylo-1,6-glucosidase.
 ACT_SITE 526 526 By similarity.
 ACT_SITE 529 529 By similarity.
 ACT_SITE 627 627 By similarity.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Glycogen biosynthesis; Glycogen storage disease; Glycosidase; Glycosyltransferase; Hydrolase; Multifunctional enzyme; Polymorphism; Reference proteome; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1532 AA 
Protein Sequence
MGHSKQIRIL LLNEMEKLEK TLFRLEQGYE LQFRLGPTLQ GKAVTVYTNY PFPGETFNRE 60
KFRSLDWENP TEREDDSDKY CKLNLQQSGS FQYYFLQGNE KSGGGYIVVD PILRVGADNH 120
VLPLDCVTLQ TFLAKCLGPF DEWESRLRVA KESGYNMIHF TPLQTLGLSR SCYSLANQLE 180
LNPDFSRPNR KYTWNDVGQL VEKLKKEWNV ICITDVVYNH TAANSKWIQE HPECAYNLVN 240
SPHLKPAWVL DRALWRFSCD VAEGKYKEKG IPALIENDHH MNSIRKIIWE DIFPKLKLWE 300
FFQVDVNKAV EQFRRLLTQE NRRVTKSDPN QHLTIIQDPE YRRFGCTVDM NIALTTFIPH 360
DKGPAAIEEC CNWFHKRMEE LNSEKHRLIN YHQEQAVNCL LGNVFYERLA GHGPKLGPVT 420
RKHPLVTRYF TFPFEEIDFS MEESMIHLPN KACFLMAHNG WVMGDDPLRN FAEPGSEVYL 480
RRELICWGDS VKLRYGNKPE DCPYLWAHMK KYTEITATYF QGVRLDNCHS TPLHVAEYML 540
DAARNLQPNL YVVAELFTGS EDLDNVFVTR LGISSLIREA MSAYNSHEEG RLVYRYGGEP 600
VGSFVQPCLR PLMPAIAHAL FMDITHDNEC PIVHRSAYDA LPSTTIVSMA CCASGSTRGY 660
DELVPHQISV VSEERFYTKW NPEALPSNTG EVNFQSGIIA ARCAISKLHQ ELGAKGFIQV 720
YVDQVDEDIV AVTRHSPSIH QSVVAVSRTA FRNPKTSFYS KEVPQMCIPG KIEEVVLEAR 780
TIERNTKPYR KDENSINGTP DITVEIREHI QLNESKIVKQ AGVATKGPNE YIQEIEFENL 840
SPGSVIIFRV SLDPHAQVAV GILRNHLTQF SPHFKSGSLA VDNADPILKI PFASLASRLT 900
LAELNQILYR CESEEKEDGG GCYDIPNWSA LKYAGLQGLM SVLAEIRPKN DLGHPFCNNL 960
RSGDWMIDYV SNRLISRSGT IAEVGKWLQA MFFYLKQIPR YLIPCYFDAI LIGAYTTLLD 1020
TAWKQMSSFV QNGSTFVKHL SLGSVQLCGV GKFPSLPILS PALMDVPYRL NEITKEKEQC 1080
CVSLAAGLPH FSSGIFRCWG RDTFIALRGI LLITGRYVEA RNIILAFAGT LRHGLIPNLL 1140
GEGIYARYNC RDAVWWWLQC IQDYCKMVPN GLDILKCPVS RMYPTDDSAP LPAGTLDQPL 1200
FEVIQEAMQK HMQGIQFRER NAGPQIDRNM KDEGFNITAG VDEETGFVYG GNRFNCGTWM 1260
DKMGESDRAR NRGIPATPRD GSAVEIVGLS KSAVRWLLEL SKKNIFPYHE VTVKRHGKAI 1320
KVSYDEWNRK IQDNFEKLFH VSEDPSDLNE KHPNLVHKRG IYKDSYGASS PWCDYQLRPN 1380
FTIAMVVAPE LFTTEKAWKA LEIAEKKLLG PLGMKTLDPD DMVYCGIYDN ALDNDNYNLA 1440
KGFNYHQGPE WLWPIGYFLR AKLYFSRLMG PETTAKTIVL VKNVLSRHYV HLERSPWKGL 1500
PELTNENAQY CPFSCETQAW SIATILETLY DL 1532 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016234; C:inclusion body; IEA:Compara.
 GO:0043033; C:isoamylase complex; TAS:ProtInc.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0016529; C:sarcoplasmic reticulum; IEA:Compara.
 GO:0004134; F:4-alpha-glucanotransferase activity; IBA:RefGenome.
 GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IBA:RefGenome.
 GO:0030247; F:polysaccharide binding; IEA:Compara.
 GO:0006006; P:glucose metabolic process; TAS:Reactome.
 GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
 GO:0005980; P:glycogen catabolic process; IBA:RefGenome.
 GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR008928; 6-hairpin_glycosidase-like.
 IPR010401; GDE_C.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR006421; Glycogen_debranch_met.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF06202; GDE_C 
SMART
  
PROSITE
  
PRINTS