CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006525
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Copper-transporting ATPase 2 
Protein Synonyms/Alias
 Copper pump 2; Wilson disease-associated protein; WND/140 kDa 
Gene Name
 ATP7B 
Gene Synonyms/Alias
 PWD; WC1; WND 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
477HAPDILAKSPQSTRAubiquitination[1]
489TRAVAPQKCFLQIKGubiquitination[2, 3]
607ATSKALVKFDPEIIGubiquitination[2, 3]
787LEHLAKSKTSEALAKubiquitination[1]
910VEEAQMSKAPIQQLAubiquitination[1]
1028IKTVMFDKTGTITHGubiquitination[2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. 
Sequence Annotation
 DOMAIN 59 125 HMA 1.
 DOMAIN 144 210 HMA 2.
 DOMAIN 258 327 HMA 3.
 DOMAIN 360 426 HMA 4.
 DOMAIN 489 555 HMA 5.
 DOMAIN 565 631 HMA 6.
 ACT_SITE 1027 1027 4-aspartylphosphate intermediate (By
 METAL 69 69 Copper 1.
 METAL 72 72 Copper 1.
 METAL 154 154 Copper 2.
 METAL 157 157 Copper 2.
 METAL 268 268 Copper 3.
 METAL 271 271 Copper 3.
 METAL 370 370 Copper 4.
 METAL 373 373 Copper 4.
 METAL 499 499 Copper 5.
 METAL 502 502 Copper 5.
 METAL 575 575 Copper 6.
 METAL 578 578 Copper 6.
 METAL 1267 1267 Magnesium (By similarity).
 METAL 1271 1271 Magnesium (By similarity).  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Copper; Copper transport; Cytoplasm; Disease mutation; Golgi apparatus; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Mitochondrion; Nucleotide-binding; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1465 AA 
Protein Sequence
MPEQERQITA REGASRKILS KLSLPTRAWE PAMKKSFAFD NVGYEGGLDG LGPSSQVATS 60
TVRILGMTCQ SCVKSIEDRI SNLKGIISMK VSLEQGSATV KYVPSVVCLQ QVCHQIGDMG 120
FEASIAEGKA ASWPSRSLPA QEAVVKLRVE GMTCQSCVSS IEGKVRKLQG VVRVKVSLSN 180
QEAVITYQPY LIQPEDLRDH VNDMGFEAAI KSKVAPLSLG PIDIERLQST NPKRPLSSAN 240
QNFNNSETLG HQGSHVVTLQ LRIDGMHCKS CVLNIEENIG QLLGVQSIQV SLENKTAQVK 300
YDPSCTSPVA LQRAIEALPP GNFKVSLPDG AEGSGTDHRS SSSHSPGSPP RNQVQGTCST 360
TLIAIAGMTC ASCVHSIEGM ISQLEGVQQI SVSLAEGTAT VLYNPSVISP EELRAAIEDM 420
GFEASVVSES CSTNPLGNHS AGNSMVQTTD GTPTSVQEVA PHTGRLPANH APDILAKSPQ 480
STRAVAPQKC FLQIKGMTCA SCVSNIERNL QKEAGVLSVL VALMAGKAEI KYDPEVIQPL 540
EIAQFIQDLG FEAAVMEDYA GSDGNIELTI TGMTCASCVH NIESKLTRTN GITYASVALA 600
TSKALVKFDP EIIGPRDIIK IIEEIGFHAS LAQRNPNAHH LDHKMEIKQW KKSFLCSLVF 660
GIPVMALMIY MLIPSNEPHQ SMVLDHNIIP GLSILNLIFF ILCTFVQLLG GWYFYVQAYK 720
SLRHRSANMD VLIVLATSIA YVYSLVILVV AVAEKAERSP VTFFDTPPML FVFIALGRWL 780
EHLAKSKTSE ALAKLMSLQA TEATVVTLGE DNLIIREEQV PMELVQRGDI VKVVPGGKFP 840
VDGKVLEGNT MADESLITGE AMPVTKKPGS TVIAGSINAH GSVLIKATHV GNDTTLAQIV 900
KLVEEAQMSK APIQQLADRF SGYFVPFIII MSTLTLVVWI VIGFIDFGVV QRYFPNPNKH 960
ISQTEVIIRF AFQTSITVLC IACPCSLGLA TPTAVMVGTG VAAQNGILIK GGKPLEMAHK 1020
IKTVMFDKTG TITHGVPRVM RVLLLGDVAT LPLRKVLAVV GTAEASSEHP LGVAVTKYCK 1080
EELGTETLGY CTDFQAVPGC GIGCKVSNVE GILAHSERPL SAPASHLNEA GSLPAEKDAV 1140
PQTFSVLIGN REWLRRNGLT ISSDVSDAMT DHEMKGQTAI LVAIDGVLCG MIAIADAVKQ 1200
EAALAVHTLQ SMGVDVVLIT GDNRKTARAI ATQVGINKVF AEVLPSHKVA KVQELQNKGK 1260
KVAMVGDGVN DSPALAQADM GVAIGTGTDV AIEAADVVLI RNDLLDVVAS IHLSKRTVRR 1320
IRINLVLALI YNLVGIPIAA GVFMPIGIVL QPWMGSAAMA ASSVSVVLSS LQLKCYKKPD 1380
LERYEAQAHG HMKPLTASQV SVHIGMDDRW RDSPRATPWD QVSYVSQVSL SSLTSDKPSR 1440
HSAAADDDGD KWSLLLNGRD EEQYI 1465 
Gene Ontology
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0005887; C:integral to plasma membrane; TAS:UniProtKB.
 GO:0005770; C:late endosome; IDA:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; IEA:Compara.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0005507; F:copper ion binding; IDA:UniProtKB.
 GO:0004008; F:copper-exporting ATPase activity; NAS:UniProtKB.
 GO:0006878; P:cellular copper ion homeostasis; TAS:UniProtKB.
 GO:0006882; P:cellular zinc ion homeostasis; IEA:Compara.
 GO:0015677; P:copper ion import; IDA:UniProtKB.
 GO:0015680; P:intracellular copper ion transport; IEA:Compara.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0046688; P:response to copper ion; IDA:UniProtKB.
 GO:0051208; P:sequestering of calcium ion; IDA:UniProtKB. 
Interpro
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR018303; ATPase_P-typ_P_site.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR027256; Cation_transp_P-typ_ATPase_IB.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom.
 IPR017969; Heavy-metal-associated_CS.
 IPR006121; HeavyMe-assoc_HMA.
 IPR006122; HMA_Cu_ion-bd. 
Pfam
 PF00122; E1-E2_ATPase
 PF00403; HMA
 PF00702; Hydrolase 
SMART
  
PROSITE
 PS00154; ATPASE_E1_E2
 PS01047; HMA_1
 PS50846; HMA_2 
PRINTS
 PR00119; CATATPASE.