CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019305
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tonsoku-like protein 
Protein Synonyms/Alias
 Inhibitor of kappa B-related protein; I-kappa-B-related protein; IkappaBR; NF-kappa-B inhibitor-like protein 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2 
Gene Name
 TONSL 
Gene Synonyms/Alias
 IKBR; NFKBIL2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
187LCNDYFRKSIFLAEQubiquitination[1]
275AYRLGSQKPVQRAAIubiquitination[1, 2]
323QLGDLFSKAGDFPRAubiquitination[3]
336RAAEAYQKQLRFAELubiquitination[1]
416LLAPCFQKALSCAQQubiquitination[1]
1245PVFRYLAKEGCALAHubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork- associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold. 
Sequence Annotation
 REPEAT 27 60 TPR 1.
 REPEAT 67 100 TPR 2.
 REPEAT 107 147 TPR 3.
 REPEAT 162 195 TPR 4.
 REPEAT 202 235 TPR 5.
 REPEAT 242 275 TPR 6.
 REPEAT 311 344 TPR 7.
 REPEAT 352 385 TPR 8.
 REPEAT 528 557 ANK 1.
 REPEAT 561 590 ANK 2.
 REPEAT 597 626 ANK 3.
 REPEAT 1069 1093 LRR 1.
 REPEAT 1097 1122 LRR 2.
 REPEAT 1128 1151 LRR 3.
 REPEAT 1188 1212 LRR 4.
 REPEAT 1247 1270 LRR 5.
 REPEAT 1275 1300 LRR 6.
 REPEAT 1331 1354 LRR 7.
 MOD_RES 719 719 Phosphoserine.  
Keyword
 Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; DNA damage; DNA repair; Leucine-rich repeat; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1378 AA 
Protein Sequence
MSLERELRQL SKAKAKAQRA GQRREEAALC HQLGELLAGH GRYAEALEQH WQELQLRERA 60
DDPLGCAVAH RKIGERLAEM EDYPAALQHQ HQYLELAHSL RNHTELQRAW ATIGRTHLDI 120
YDHCQSRDAL LQAQAAFEKS LAIVDEELEG TLAQGELNEM RTRLYLNLGL TFESLQQTAL 180
CNDYFRKSIF LAEQNHLYED LFRARYNLGT IHWRAGQHSQ AMRCLEGARE CAHTMRKRFM 240
ESECCVVIAQ VLQDLGDFLA AKRALKKAYR LGSQKPVQRA AICQNLQHVL AVVRLQQQLE 300
EAEGRDPQGA MVICEQLGDL FSKAGDFPRA AEAYQKQLRF AELLDRPGAE RAIIHVSLAT 360
TLGDMKDHHG AVRHYEEELR LRSGNVLEEA KTWLNIALSR EEAGDAYELL APCFQKALSC 420
AQQAQRPQLQ RQVLQHLHTV QLRLQPQEAP ETETRLRELS VAEDEDEEEE AEEAAATAES 480
EALEAGEVEL SEGEDDTDGL TPQLEEDEEL QGHLGRRKGS KWNRRNDMGE TLLHRACIEG 540
QLRRVQDLVR QGHPLNPRDY CGWTPLHEAC NYGHLEIVRF LLDHGAAVDD PGGQGCEGIT 600
PLHDALNCGH FEVAELLLER GASVTLRTRK GLSPLETLQQ WVKLYRRDLD LETRQKARAM 660
EMLLQAAASG QDPHSSQAFH TPSSLLFDPE TSPPLSPCPE PPSNSTRLPE ASQAHVRVSP 720
GQAAPAMARP RRSRHGPASS SSSSEGEDSA GPARPSQKRP RCSATAQRVA AWTPGPASNR 780
EAATASTSRA AYQAAIRGVG SAQSRLGPGP PRGHSKALAP QAALIPEEEC LAGDWLELDM 840
PLTRSRRPRP RGTGDNRRPS STSGSDSEES RPRARAKQVR LTCMQSCSAP VNAGPSSLAS 900
EPPGSPSTPR VSEPSGDSSA AGQPLGPAPP PPIRVRVQVQ DHLFLIPVPH SSDTHSVAWL 960
AEQAAQRYYQ TCGLLPRLTL RKEGALLAPQ DLIPDVLQSN DEVLAEVTSW DLPPLTDRYR 1020
RACQSLGQGE HQQVLQAVEL QGLGLSFSAC SLALDQAQLT PLLRALKLHT ALRELRLAGN 1080
RLGDKCVAEL VAALGTMPSL ALLDLSSNHL GPEGLRQLAM GLPGQATLQS LEELDLSMNP 1140
LGDGCGQSLA SLLHACPLLS TLRLQACGFG PSFFLSHQTA LGSAFQDAEH LKTLSLSYNA 1200
LGAPALARTL QSLPAGTLLH LELSSVAAGK GDSDLMEPVF RYLAKEGCAL AHLTLSANHL 1260
GDKAVRDLCR CLSLCPSLIS LDLSANPEIS CASLEELLST LQKRPQGLSF LGLSGCAVQG 1320
PLGLGLWDKI AAQLRELQLC SRRLCAEDRD ALRQLQPSRP GPGECTLDHG SKLFFRRL 1378 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0043596; C:nuclear replication fork; IDA:UniProtKB.
 GO:0042393; F:histone binding; IDA:UniProtKB.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0042994; P:cytoplasmic sequestering of transcription factor; TAS:ProtInc.
 GO:0000724; P:double-strand break repair via homologous recombination; IMP:UniProtKB.
 GO:0031297; P:replication fork processing; IMP:UniProtKB. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00023; Ank 
SMART
 SM00248; ANK
 SM00028; TPR 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS51450; LRR
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS
 PR01415; ANKYRIN.