UniProt Accession

 SORT_HUMAN; Q99523; B4DWI3; C0JYZ0; Q8IZ49 

Genbank Protein ID

 X98248; FJ525881; AK301548; AL390252; BC023542 

Genbank Nucleotide ID

 CAA66904.2; ACN81319.1; BAG63045.1; CAI13180.1; AAH23542.1 

Protein Name

 Sortilin 

Protein Synonyms/Alias

 100 kDa NT receptor; Glycoprotein 95; Gp95; Neurotensin receptor 3; NT3; NTR3 

Gene Name

 SORT1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
152	YRSEDYGKNFKDITD	ubiquitination	[1]
294	WRTSDLGKSFKTIGV	ubiquitination	[2]
388	DRGIVYSKSLDRHLY	ubiquitination	[1, 2]
818	DTASHTNKSGYHDDS	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. 

Sequence Annotation

 REPEAT 145 156 BNR 1.
 REPEAT 198 209 BNR 2.
 REPEAT 240 251 BNR 3.
 REPEAT 287 298 BNR 4.
 REPEAT 328 339 BNR 5.
 REPEAT 377 388 BNR 6.
 REPEAT 428 439 BNR 7.
 REPEAT 506 517 BNR 8.
 REPEAT 548 559 BNR 9.
 REGION 50 61 Intrachain binding of the propeptide and
 REGION 612 756 Interactions with LRPAP1 and NGFB.
 REGION 779 831 Golgi to endosome transport and
 MOTIF 787 792 Endocytosis signal (Probable).
 MOD_RES 819 819 Phosphoserine.
 MOD_RES 825 825 Phosphoserine.
 CARBOHYD 98 98 N-linked (GlcNAc...) (Probable).
 CARBOHYD 162 162 N-linked (GlcNAc...).
 CARBOHYD 163 163 N-linked (GlcNAc...); atypical.
 CARBOHYD 274 274 N-linked (GlcNAc...) (Potential).
 CARBOHYD 406 406 N-linked (GlcNAc...).
 CARBOHYD 582 582 N-linked (GlcNAc...).
 CARBOHYD 684 684 N-linked (GlcNAc...) (Potential).
 DISULFID 86 556
 DISULFID 257 277
 DISULFID 448 458
 DISULFID 612 651
 DISULFID 634 666
 DISULFID 668 723
 DISULFID 675 688
 DISULFID 702 740  

Keyword

 3D-structure; Alternative splicing; Cell membrane; Cleavage on pair of basic residues; Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; Disulfide bond; Endocytosis; Endoplasmic reticulum; Endosome; Glycoprotein; Golgi apparatus; Lysosome; Membrane; Nucleus; Osteogenesis; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 831 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IDA:BHF-UCL.
 GO:0030136; C:clathrin-coated vesicle; IDA:BHF-UCL.
 GO:0005905; C:coated pit; IDA:BHF-UCL.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0005769; C:early endosome; IDA:BHF-UCL.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:BHF-UCL.
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IDA:BHF-UCL.
 GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0005886; C:plasma membrane; IDA:BHF-UCL.
 GO:0030140; C:trans-Golgi network transport vesicle; IEA:Compara.
 GO:0010465; F:nerve growth factor receptor activity; IDA:BHF-UCL.
 GO:0030379; F:neurotensin receptor activity, non-G-protein coupled; IDA:BHF-UCL.
 GO:0097190; P:apoptotic signaling pathway; IMP:BHF-UCL.
 GO:0006897; P:endocytosis; IDA:BHF-UCL.
 GO:0008333; P:endosome to lysosome transport; IDA:BHF-UCL.
 GO:0032509; P:endosome transport via multivesicular body sorting pathway; IDA:BHF-UCL.
 GO:0046323; P:glucose import; IMP:BHF-UCL.
 GO:0006895; P:Golgi to endosome transport; IDA:BHF-UCL.
 GO:0014902; P:myotube differentiation; IMP:BHF-UCL.
 GO:0043066; P:negative regulation of apoptotic process; IDA:BHF-UCL.
 GO:0051005; P:negative regulation of lipoprotein lipase activity; IDA:BHF-UCL.
 GO:0007218; P:neuropeptide signaling pathway; IDA:BHF-UCL.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; IMP:BHF-UCL.
 GO:0001503; P:ossification; IEA:UniProtKB-KW.
 GO:0048227; P:plasma membrane to endosome transport; IDA:BHF-UCL.
 GO:0010468; P:regulation of gene expression; IMP:BHF-UCL.
 GO:0032868; P:response to insulin stimulus; IMP:BHF-UCL.
 GO:0016050; P:vesicle organization; IDA:BHF-UCL. 

Interpro

 IPR006581; VPS10.
 IPR015943; WD40/YVTN_repeat-like_dom. 

Pfam

  

SMART

 SM00602; VPS10 

PROSITE

  

PRINTS