UniProt Accession

 DKGB_ECOLI; P30863; P77777 

Genbank Protein ID

 D12650; U70214; U00096; AP009048; V00336 

Genbank Nucleotide ID

 BAA02170.1; AAB08629.1; AAC73312.1; BAA77878.2; CAA23619.1 

Protein Name

 2,5-diketo-D-gluconic acid reductase B 

Protein Synonyms/Alias

 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D 

Gene Name

 dkgB 

Gene Synonyms/Alias

 yafB; b0207; JW0197 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
72	IWIENLSKDKLIPSL	acetylation	[1]
74	IENLSKDKLIPSLKE	acetylation	[1]
80	DKLIPSLKESLQKLR	acetylation	[1]
188	LAYGKALKDEVIARI	acetylation	[1]
233	KNLESNLKAQNLQLD	acetylation	[1]
244	LQLDAEDKKAIAALD	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 

Sequence Annotation

 NP_BIND 179 231 NADP (By similarity).
 ACT_SITE 39 39 Proton donor (By similarity).
 BINDING 97 97 Substrate (By similarity).  

Keyword

 Ascorbate biosynthesis; Complete proteome; Cytoplasm; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 267 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004033; F:aldo-keto reductase (NADP) activity; IDA:EcoCyc.
 GO:0047681; F:aryl-alcohol dehydrogenase (NADP+) activity; IDA:EcoCyc.
 GO:1990002; F:methylglyoxal reductase (NADPH-dependent, acetol producing); IDA:EcoCyc.
 GO:0019853; P:L-ascorbic acid biosynthetic process; IEA:UniProtKB-KW.
 GO:0051596; P:methylglyoxal catabolic process; IMP:EcoCyc. 

Interpro

 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 

PRINTS

 PR00069; ALDKETRDTASE.