CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024797
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Brefeldin A-inhibited guanine nucleotide-exchange protein 1 
Protein Synonyms/Alias
 BIG1; Brefeldin A-inhibited GEP 1; ADP-ribosylation factor guanine nucleotide-exchange factor 1 
Gene Name
 Arfgef1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
218NQALQEAKQMERERHubiquitination[1]
754EFLGDNDKFNKEVMYubiquitination[1]
1691LESHRFAKAFNSNNEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity). 
Sequence Annotation
 DOMAIN 688 877 SEC7.
 REGION 2 224 DCB; DCB:DCB domain and DCB:HUS domain
 REGION 554 574 HUS; DCB:HUS domain interaction (By
 MOTIF 708 712 Nuclear localization signal (NLS) (By
 MOD_RES 1076 1076 Phosphoserine (By similarity).
 MOD_RES 1566 1566 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Golgi apparatus; Guanine-nucleotide releasing factor; Membrane; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1846 AA 
Protein Sequence
MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKVETEK QSPPHGEAKA 60
GSGTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL DCLQKLIAYG HLTGRAPDST 120
TPGKKLIDRI IETICGCFQG PQTDEGVQLQ IIKALLTAVT SQHIEIHEGT VLQAVRTCYN 180
IYLASKNLIN QTTAKATLTQ MLNVIFARME NQALQEAKQM ERERHRQQQH LLQSPVSHHE 240
PESPHLRYLP PQTVDHINQE HEGDLEPQTH DVDKSLQDDT EPENGSDISS AENEQTEADQ 300
ATAAETLSKN DILYDGDYEE KPLDIVQSIV EEMVNIIVGD MGEGMAISAS TEGNTGTVED 360
GSDSENIQAN GIPGTPISVA YTPSLPDDRL SVSSNDTQES GNSSGPSPGA KFSHILQKDA 420
FLVFRSLCKL SMKPLSDGPP DPKSHELRSK ILSLQLLLSI LQNAGPVFRT NEMFINAIKQ 480
YLCVALSKNG VSSVPEVFEL SLSIFLTLLS NFKTHLKMQI EVFFKEIFLY ILETSTSSFD 540
HKWMVIQTLT RICADAQSVV DIYVNYDCDL NAANIFERLV NDLSKIAQGR GSQELGMSNV 600
QELSLRKKGL ECLVSILKCM VEWSKDQYVN PNSQTTLGQE KPSEQEISEV KHPETINRYG 660
SLNSLESTSS SGIGSYSTQM SGTDNPEQFE VLKQQKEIIE QGIDLFNKKP KRGIQYLQEQ 720
GMLGTTPEDI AQFLHQEERL DSTQVGEFLG DNDKFNKEVM YAYVDQHDFS GKDFVSALRL 780
FLEGFRLPGE AQKIDRLMEK FAARYLECNQ GQTLFASADT AYVLAYSIIM LTTDLHSPQV 840
KNKMTKEQYI KMNRGINDSK DLPEEYLSAI YNEIAGKKIS MKETKELTIP TKSTKQNVAS 900
EKQRRLLYNL EMEQMAKTAK ALMEAVSHVQ APFTSATHLE HVRPMFKLAW TPFLAAFSVG 960
LQDCDDTEVA SLCLEGIRCA IRIACIFSIQ LERDAYVQAL ARFTLLTVSS GITEMKQKNI 1020
DTIKTLITVA HTDGNYLGNS WHEILKCISQ LELAQLIGTG VKPRYISGTV RGREGSLTGT 1080
KDQAPDEFVG LGLVGGNVDW KQIASIQESI GETSSQSVVV AVDRIFTGST RLDGNAIVDF 1140
VRWLCAVSMD ELLSTTHPRM FSLQKIVEIS YYNMGRIRLQ WSRIWEVIGD HFNKVGCNPN 1200
EDVAIFAVDS LRQLSMKFLE KGELANFRFQ KDFLRPFEHI MKRNRSPTIR DMVVRCIAQM 1260
VNSQAANIRS GWKNIFSVFH LAASDQDESI VELAFQTTGH IVTLVFEKHF PATIDSFQDA 1320
VKCLSEFACN AAFPDTSMEA IRLIRHCAKY VSDRPQAFKE YTSDDMSVAP EDRVWVRGWF 1380
PILFELSCII NRCKLDVRTR GLTVMFEIMK TYGHTYEKHW WQDLFRIVFR IFDNMKLPEQ 1440
QTEKAEWMTT TCNHALYAIC DVFTQYLEVL SDVLLDDIFA QLYWCVQQDN EQLARSGTNC 1500
LENVVILNGE KFTLEIWDKT CNCTLDIFKT TIPHALLTWR PTSGEAEPPS PSAVSEKPLD 1560
AISQKSVDIH DSIQPRSSDN RQQAPLVSVS TVSEEVSKVK STAKFPEQKL FAALLIKCVV 1620
QLELIQTIDN IVFFPATSKK EDAENLAAAQ RDAVDFDVRV DTQDQGMYRF LTSQQLFKLL 1680
DCLLESHRFA KAFNSNNEQR TALWKAGFKG KSKPNLLKQE TSSLACGLRI LFRMYMDESR 1740
VSAWEEVQQR LLNVCREALS YFLTLTSESH REAWTNLLLL FLTKVLKISD SRFKAHASFY 1800
YPLLCEIMQF DLIPELRAVL RRFFLRIGIV FQISQPPEQE LGINRQ 1846 
Gene Ontology
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0000139; C:Golgi membrane; ISS:UniProtKB.
 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030532; C:small nuclear ribonucleoprotein complex; ISS:UniProtKB.
 GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
 GO:0005086; F:ARF guanyl-nucleotide exchange factor activity; IBA:RefGenome.
 GO:0034237; F:protein kinase A regulatory subunit binding; ISS:UniProtKB.
 GO:0010256; P:endomembrane system organization; ISS:UniProtKB.
 GO:0007030; P:Golgi organization; ISS:UniProtKB.
 GO:0030837; P:negative regulation of actin filament polymerization; ISS:UniProtKB.
 GO:0034259; P:negative regulation of Rho GTPase activity; ISS:UniProtKB.
 GO:0090284; P:positive regulation of protein glycosylation in Golgi; ISS:UniProtKB.
 GO:0090303; P:positive regulation of wound healing; ISS:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
 GO:2000114; P:regulation of establishment of cell polarity; ISS:UniProtKB.
 GO:0016192; P:vesicle-mediated transport; IBA:RefGenome. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR015403; DUF1981_SEC7_assoc.
 IPR000904; Sec7.
 IPR023394; SEC7_alpha_orthog. 
Pfam
 PF09324; DUF1981
 PF01369; Sec7 
SMART
 SM00222; Sec7 
PROSITE
 PS50190; SEC7 
PRINTS