UniProt Accession

 LCAP_HUMAN; Q9UIQ6; O00769; Q15145; Q59H76; Q9TNQ2; Q9TNQ3; Q9UIQ7 

Genbank Protein ID

 D50810; U62768; U62769; AJ131023; AJ131025; AJ131026; AJ131027; AJ131028; AJ131029; AJ131030; AJ131031; AJ131032; AJ131033; AJ131034; AJ131035; AJ131036; AJ131037; AJ131038; AJ131039; AJ131025; AJ131026; AJ131027; AJ131028; AJ131029; AJ131030; AJ131031; AJ131032; AJ131033; AJ131034; AJ131035; AJ131036; AJ131037; AJ131038; AJ131039; AB208883 

Genbank Nucleotide ID

 BAA09436.1; AAB66672.1; AAB66673.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB61646.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; CAB94753.1; BAD92120.1 

Protein Name

 Leucyl-cystinyl aminopeptidase 

Protein Synonyms/Alias

 Cystinyl aminopeptidase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; IRAP; Oxytocinase; OTase; Placental leucine aminopeptidase; P-LAP; Leucyl-cystinyl aminopeptidase, pregnancy serum form 

Gene Name

 LNPEP 

Gene Synonyms/Alias

 OTASE 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
75	EDYESSAKLLGMSFM	ubiquitination	[1, 2]
159	QPFATNGKLFPWAQI	ubiquitination	[2]
287	TDESNEKKYFAATQF	ubiquitination	[2]
608	LDVKRMMKTWTLQKG	ubiquitination	[2]
626	VTVQKKGKELFIQQE	ubiquitination	[2]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. 

Sequence Annotation

 REGION 96 101 Tankyrase binding.
 REGION 428 432 Substrate binding (By similarity).
 MOTIF 53 54 Dileucine internalization motif
 MOTIF 76 77 Dileucine internalization motif
 ACT_SITE 465 465 Proton acceptor (By similarity).
 METAL 464 464 Zinc; catalytic (By similarity).
 METAL 468 468 Zinc; catalytic (By similarity).
 METAL 487 487 Zinc; catalytic (By similarity).
 BINDING 295 295 Substrate (By similarity).
 MOD_RES 80 80 Phosphoserine (By similarity).
 MOD_RES 91 91 Phosphoserine (By similarity).
 CARBOHYD 145 145 N-linked (GlcNAc...) (Potential).
 CARBOHYD 184 184 N-linked (GlcNAc...).
 CARBOHYD 215 215 N-linked (GlcNAc...) (Potential).
 CARBOHYD 256 256 N-linked (GlcNAc...) (Potential).
 CARBOHYD 266 266 N-linked (GlcNAc...) (Potential).
 CARBOHYD 368 368 N-linked (GlcNAc...) (Potential).
 CARBOHYD 374 374 N-linked (GlcNAc...) (Potential).
 CARBOHYD 448 448 N-linked (GlcNAc...).
 CARBOHYD 525 525 N-linked (GlcNAc...) (Potential).
 CARBOHYD 578 578 N-linked (GlcNAc...) (Potential).
 CARBOHYD 598 598 N-linked (GlcNAc...) (Potential).
 CARBOHYD 664 664 N-linked (GlcNAc...) (Potential).
 CARBOHYD 682 682 N-linked (GlcNAc...).
 CARBOHYD 760 760 N-linked (GlcNAc...) (Potential).
 CARBOHYD 834 834 N-linked (GlcNAc...) (Potential).
 CARBOHYD 850 850 N-linked (GlcNAc...).
 CARBOHYD 989 989 N-linked (GlcNAc...) (Potential).  

Keyword

 Alternative splicing; Aminopeptidase; Cell membrane; Complete proteome; Direct protein sequencing; Glycoprotein; Hydrolase; Membrane; Metal-binding; Metalloprotease; Phosphoprotein; Polymorphism; Protease; Reference proteome; Secreted; Signal-anchor; Transmembrane; Transmembrane helix; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1025 AA 

Protein Sequence

Gene Ontology

 GO:0030659; C:cytoplasmic vesicle membrane; TAS:Reactome.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031905; C:early endosome lumen; TAS:Reactome.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0004177; F:aminopeptidase activity; TAS:HGNC.
 GO:0008237; F:metallopeptidase activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; TAS:ProtInc.
 GO:0002480; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; TAS:Reactome.
 GO:0007267; P:cell-cell signaling; TAS:ProtInc.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0007565; P:female pregnancy; TAS:ProtInc.
 GO:0030163; P:protein catabolic process; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; TAS:Reactome.
 GO:0006508; P:proteolysis; TAS:ProtInc. 

Interpro

 IPR024571; DUF3358.
 IPR001930; Peptidase_M1.
 IPR014782; Peptidase_M1_N. 

Pfam

 PF11838; DUF3358
 PF01433; Peptidase_M1 

SMART

  

PROSITE

 PS00142; ZINC_PROTEASE 

PRINTS

 PR00756; ALADIPTASE.