CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000333
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aurora kinase A 
Protein Synonyms/Alias
 Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A 
Gene Name
 AURKA 
Gene Synonyms/Alias
 AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
141EIGRPLGKGKFGNVYubiquitination[1]
143GRPLGKGKFGNVYLAubiquitination[1]
250ALSYCHSKRVIHRDIubiquitination[1]
258RVIHRDIKPENLLLGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. 
Sequence Annotation
 DOMAIN 133 383 Protein kinase.
 NP_BIND 210 213 ATP.
 REGION 280 293 Activation segment.
 ACT_SITE 256 256 Proton acceptor.
 BINDING 143 143 ATP; via amide nitrogen.
 BINDING 162 162 ATP.
 BINDING 274 274 ATP.
 MOD_RES 41 41 Phosphoserine.
 MOD_RES 51 51 Phosphoserine.
 MOD_RES 287 287 Phosphothreonine.
 MOD_RES 288 288 Phosphothreonine.
 MOD_RES 342 342 Phosphoserine; by PKA and PAK.  
Keyword
 3D-structure; ATP-binding; Cell cycle; Cell division; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Microtubule; Mitosis; Nucleotide-binding; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 403 AA 
Protein Sequence
MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRVPLQ 60
AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN 120
EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILA LKVLFKAQLE KAGVEHQLRR 180
EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL 240
ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM 300
IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKR ISRVEFTFPD FVTEGARDLI 360
SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS 403 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030496; C:midbody; TAS:UniProtKB.
 GO:0005634; C:nucleus; IDA:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0005876; C:spindle microtubule; IDA:BHF-UCL.
 GO:0031616; C:spindle pole centrosome; IDA:BHF-UCL.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004712; F:protein serine/threonine/tyrosine kinase activity; TAS:UniProtKB.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; NAS:UniProtKB.
 GO:0045840; P:positive regulation of mitosis; TAS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; TAS:UniProtKB.
 GO:0046605; P:regulation of centrosome cycle; TAS:UniProtKB.
 GO:0031647; P:regulation of protein stability; IMP:UniProtKB.
 GO:0007051; P:spindle organization; NAS:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS