CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020055
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ankyrin repeat domain-containing protein 32 
Protein Synonyms/Alias
 BRCT domain-containing protein 1 
Gene Name
 ANKRD32 
Gene Synonyms/Alias
 BRCTD1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MEDGTPKHIIQMTGubiquitination[1]
36KLDCTFIKSEKYKNCubiquitination[1]
109PQMQSAPKRWREELKubiquitination[1]
150IRVLEAGKANVILPKubiquitination[1]
223HSNEETNKDFRKDAGubiquitination[2]
227ETNKDFRKDAGFLEMubiquitination[1]
235DAGFLEMKGALRETMubiquitination[1]
295SHTYENQKEIKKKDEubiquitination[1]
348NRDQKEMKNSIFAEYubiquitination[1]
357SIFAEYAKESKAMAIubiquitination[1]
379EIKNTLRKHIYRAQAubiquitination[1]
396YNCIRIDKQPVYNVEubiquitination[1]
405PVYNVEVKNAEFPRGubiquitination[2]
717SYLPALGKTGVLGSGubiquitination[1]
753MLNGTKQKQVEGLPEubiquitination[1]
788EGELSCSKENCPSVVacetylation[3]
797NCPSVVKKMNFHKTNubiquitination[4]
802VKKMNFHKTNLKGETubiquitination[4]
806NFHKTNLKGETALHRubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
 DOMAIN 12 77 BRCT 1.
 DOMAIN 119 196 BRCT 2.
 REPEAT 806 836 ANK 1.
 REPEAT 840 869 ANK 2.
 REPEAT 874 903 ANK 3.  
Keyword
 ANK repeat; Complete proteome; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1058 AA 
Protein Sequence
MEDGTPKHII QMTGFKMEEK EALVKLLLKL DCTFIKSEKY KNCTHLIAER LCKSEKFLAA 60
CAAGKWILTK DYIIHSAKSG RWLDETTYEW GYKIEKDSRY SPQMQSAPKR WREELKRTGA 120
PGAFHRWKVV LLVRTDKRSD SLIRVLEAGK ANVILPKSSP SGITHVIASN ARIKAEKEKD 180
NFKAPFYPIQ YLGDFLLEKE IQNDEDSQTN SVWTEHSNEE TNKDFRKDAG FLEMKGALRE 240
TMYRTQKEMQ NHEDVNVGSI LIQHHKKEKF SGSSKDLKFV KMRNTFGSHT YENQKEIKKK 300
DEDIQRSYTL RRKRKKGKES NCKKGVEHEK IKSTLRRHIY NRDQKEMKNS IFAEYAKESK 360
AMAIKTDVDV VEIKNTLRKH IYRAQAVRYN CIRIDKQPVY NVEVKNAEFP RGVLNLIESL 420
IEGHFFKEAI EELSTLQAHY IPPVCVLHAL LENVLQDNID TFSGRYFHIL SALLHLHPPW 480
KSPAMSRYYL ELFQCPTCMK GAWSLVEVLI RSCLFNESFC HQISENIGSK VLHLTLLKFF 540
FNLIESEVQH LSQKLYDWSD SQNLKITGKA MLLEIFWSGS ETSGLLTKPV NMLLEWTIYS 600
HKEKFKSNDV FKHELAYLLA GILGAAIDYW IFLGLKMGRN VMRHMSDDLG SYVSLSCDDF 660
SSQELEIFIC SFSSSWLQMF VAEAVFKKLC LQSSGSVSSE PLSLQKMVYS YLPALGKTGV 720
LGSGKIQVSK KIGQRPCFDS QRTLLMLNGT KQKQVEGLPE LLDLNLAKCS SSLKKLKKKS 780
EGELSCSKEN CPSVVKKMNF HKTNLKGETA LHRACINNQV EKLILLLSLP GIDINVKDNA 840
GWTPLHEACN YGNTVCVQEI LQRCPEVDLL TQVDGVTPLH DALSNGHVEI GKLLLQHGGP 900
VLLQQRNAKG ELPLDYVVSP QIKEELFAIT KIEDTVENFH AQAEKHFHYQ QLEFGSFLLS 960
RMLLNFCSIF DLSSEFILAS KGLTHLNELL MACKSHKETT SVHTDWLLDL YAGNIKTLQK 1020
LPHILKELPE NLKVCPGVHT EALMITLEMM CRSVMEFS 1058 
Gene Ontology
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR001357; BRCT_dom. 
Pfam
 PF00023; Ank
 PF12796; Ank_2 
SMART
 SM00248; ANK
 SM00292; BRCT 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50172; BRCT 
PRINTS