CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000693
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras GTPase-activating protein 4 
Protein Synonyms/Alias
 Calcium-promoted Ras inactivator; Ras p21 protein activator 4; RasGAP-activating-like protein 2 
Gene Name
 RASA4 
Gene Synonyms/Alias
 CAPRI; GAPL; KIAA0538 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
569SLQAPPVKEGPLFIHubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Ca(2+)-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis. 
Sequence Annotation
 DOMAIN 1 88 C2 1.
 DOMAIN 129 216 C2 2.
 DOMAIN 302 512 Ras-GAP.
 DOMAIN 566 673 PH.
 ZN_FING 675 711 Btk-type.  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; GTPase activation; Membrane; Metal-binding; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 803 AA 
Protein Sequence
MAKRSSLYIR IVEGKNLPAK DITGSSDPYC IVKVDNEPII RTATVWKTLC PFWGEEYQVH 60
LPPTFHAVAF YVMDEDALSR DDVIGKVCLT RDTIASHPKG FSGWAHLTEV DPDEEVQGEI 120
HLRLEVWPGA RACRLRCSVL EARDLAPKDR NGTSDPFVRV RYKGRTRETS IVKKSCYPRW 180
NETFEFELQE GAMEALCVEA WDWDLVSRND FLGKVVIDVQ RLRVVQQEEG WFRLQPDQSK 240
SRRHDEGNLG SLQLEVRLRD ETVLPSSYYQ PLVHLLCHEV KLGMQGPGQL IPLIEETTST 300
ECRQDVATNL LKLFLGQGLA KDFLDLLFQL ELSRTSETNT LFRSNSLASK SMESFLKVAG 360
MQYLHGVLGP IINKVFEEKK YVELDPSKVE VKDVGCSGLH RPQTEAEVLE QSAQTLRAHL 420
GALLSALSRS VRACPAVVRA TFRQLFRRVR ERFPGAQHEN VPFIAVTSFL CLRFFSPAIM 480
SPKLFHLRER HADARTSRTL LLLAKAVQNV GNMDTPASRA KEAWMEPLQP TVRQGVAQLK 540
DFITKLVDIE EKDELDLQRT LSLQAPPVKE GPLFIHRTKG KGPLMSSSFK KLYFSLTTEA 600
LSFAKTPSSK KSALIKLANI RAAEKVEEKS FGGSHVMQVI YTDDAGRPQT AYLQCKCVNE 660
LNQWLSALRK VSINNTGLLG SYHPGVFRGD KWSCCHQKEK TGQGCDKTRS RVTLQEWNDP 720
LDHDLEAQLI YRHLLGVEAM LWERHRELSG GAEAGTVPTS PGKVPEDSLA RLLRVLQDLR 780
EAHSSSPAGS PPSEPNCLLE LQT 803 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:RefGenome.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0031235; C:intrinsic to internal side of plasma membrane; ISS:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005099; F:Ras GTPase activator activity; ISS:RefGenome.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0046580; P:negative regulation of Ras protein signal transduction; ISS:RefGenome. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR020477; C2_dom.
 IPR018029; C2_membr_targeting.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001936; RasGAP.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot.
 IPR001562; Znf_Btk_motif. 
Pfam
 PF00779; BTK
 PF00168; C2
 PF00616; RasGAP 
SMART
 SM00107; BTK
 SM00239; C2
 SM00233; PH
 SM00323; RasGAP 
PROSITE
 PS50004; C2
 PS50003; PH_DOMAIN
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2
 PS51113; ZF_BTK 
PRINTS
 PR00360; C2DOMAIN.