CPLM-002677

Genbank Nucleotide ID

Cytochrome c1, heme protein, mitochondrial

Protein Synonyms/Alias

Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; Cytochrome c-1

Homo sapiens (Human)

Position	Peptide	Type	References
50	QAVALSSKSGLSRGR	ubiquitination	[1, 2, 3, 4, 5, 6]
170	EMFMRPGKLFDYFPK	ubiquitination	[1]
177	KLFDYFPKPYPNSEA	ubiquitination	[1, 2, 3, 4, 5, 6, 7]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

DOMAIN 108 209 Cytochrome c.
METAL 125 125 Iron (heme axial ligand).
METAL 244 244 Iron (heme axial ligand) (By similarity).
BINDING 121 121 Heme (covalent).
BINDING 124 124 Heme (covalent).

Complete proteome; Electron transport; Heme; Iron; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Polymorphism; Reference proteome; Respiratory chain; Transit peptide; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030054; C:cell junction; IDA:HPA.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
GO:0070469; C:respiratory chain; IEA:UniProtKB-KW.
GO:0045155; F:electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; TAS:ProtInc.
GO:0020037; F:heme binding; IEA:InterPro.
GO:0005506; F:iron ion binding; IEA:InterPro.
GO:0022904; P:respiratory electron transport chain; TAS:Reactome.
GO:0044281; P:small molecule metabolic process; TAS:Reactome.

IPR002326; Cyt_c1.
IPR021157; Cyt_c1_TM_anchor_C.
IPR009056; Cyt_c_dom.

PF02167; Cytochrom_C1

PR00603; CYTOCHROMEC1.