CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022497
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 7 
Protein Synonyms/Alias
 Cell migration-inducing protein 23 
Gene Name
 TBC1D7 
Gene Synonyms/Alias
 HSPC239; TBC7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16FRSVYYEKVGFRGVEubiquitination[1]
82AKVMMYRKEQYLDVLubiquitination[1]
93LDVLHALKVVRFVSDubiquitination[1]
118MYQLESGKLPRSPSFubiquitination[1, 2]
165FVNQLNTKYRDSLPQubiquitination[1, 2]
175DSLPQLPKAFEQYLNubiquitination[1]
200RMCSAAPKLPYDLWFubiquitination[1]
208LPYDLWFKRCFAGCLubiquitination[1, 2, 3, 4]
261NSAEKITKFLENIPQubiquitination[1, 2]
277SSDAIVSKAIDLWHKubiquitination[1, 2]
284KAIDLWHKHCGTPVHubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of the TSC-TBC complex, that contains TBC1D7 in addition to the TSC1-TSC2 complex and consists of the functional complex possessing GTPase-activating protein (GAP) activity toward RHEB in response to alterations in specific cellular growth conditions. The small GTPase RHEB is a direct activator of the protein kinase activity of mTORC1 and the TSC-TBC complex acts as a negative regulator of mTORC1 signaling cascade by acting as a GAP for RHEB. Participates in the proper sensing of growth factors and glucose, but not amino acids, by mTORC1. It is unclear whether TBC1D7 acts as a GTPase-activating protein and additional studies are required to answer this question. 
Sequence Annotation
 DOMAIN 50 231 Rab-GAP TBC.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasmic vesicle; GTPase activation; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 293 AA 
Protein Sequence
MTEDSQRNFR SVYYEKVGFR GVEEKKSLEI LLKDDRLDTE KLCTFSQRFP LPSMYRALVW 60
KVLLGILPPH HESHAKVMMY RKEQYLDVLH ALKVVRFVSD ATPQAEVYLR MYQLESGKLP 120
RSPSFPLEPD DEVFLAIAKA MEEMVEDSVD CYWITRRFVN QLNTKYRDSL PQLPKAFEQY 180
LNLEDGRLLT HLRMCSAAPK LPYDLWFKRC FAGCLPESSL QRVWDKVVSG SCKILVFVAV 240
EILLTFKIKV MALNSAEKIT KFLENIPQDS SDAIVSKAID LWHKHCGTPV HSS 293 
Gene Ontology
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0031410; C:cytoplasmic vesicle; IDA:UniProtKB.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0032862; P:activation of Rho GTPase activity; IMP:UniProtKB.
 GO:0032007; P:negative regulation of TOR signaling cascade; IMP:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC.
 GO:0070848; P:response to growth factor stimulus; IMP:UniProtKB. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
  
PROSITE
 PS50086; TBC_RABGAP 
PRINTS