CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017557
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 38 
Protein Synonyms/Alias
 Deubiquitinating enzyme 38; HP43.8KD; Ubiquitin thioesterase 38; Ubiquitin-specific-processing protease 38 
Gene Name
 USP38 
Gene Synonyms/Alias
 KIAA1891 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
162VQCIPKGKLSITFCQubiquitination[1]
439CLSRLSGKSETGKTGubiquitination[2]
486NGCNSLMKKLQHLFAubiquitination[2]
541DRLHEEEKILKVQASubiquitination[1]
588GEKTLIEKMFGGKLRubiquitination[1]
773LRFSYDQKYHVRRKIubiquitination[1, 3, 4]
779QKYHVRRKILDNVSLubiquitination[1]
795LVLELPVKRITSFSSubiquitination[1]
825ENLAKKLKPSGTDEAubiquitination[1]
907FEQDLENKEMSKEWFubiquitination[2]
982ELMDAITKDNKLYLQubiquitination[1]
985DAITKDNKLYLQEQEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Deubiquitinating enzyme exhibiting a preference towards 'Lys-63'-linked Ubiquitin chains. 
Sequence Annotation
 ACT_SITE 454 454 Nucleophile (By similarity).
 ACT_SITE 857 857 Proton acceptor (By similarity).  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1042 AA 
Protein Sequence
MDKILEGLVS SSHPLPLKRV IVRKVVESAE HWLDEAQCEA MFDLTTRLIL EGQDPFQRQV 60
GHQVLEAYAR YHRPEFESFF NKTFVLGLLH QGYHSLDRKD VAILDYIHNG LKLIMSCPSV 120
LDLFSLLQVE VLRMVCERPE PQLCARLSDL LTDFVQCIPK GKLSITFCQQ LVRTIGHFQC 180
VSTQERELRE YVSQVTKVSN LLQNIWKAEP ATLLPSLQEV FASISSTDAS FEPSVALASL 240
VQHIPLQMIT VLIRSLTTDP NVKDASMTQA LCRMIDWLSW PLAQHVDTWV IALLKGLAAV 300
QKFTILIDVT LLKIELVFNR LWFPLVRPGA LAVLSHMLLS FQHSPEAFHL IVPHVVNLVH 360
SFKNDGLPSS TAFLVQLTEL IHCMMYHYSG FPDLYEPILE AIKDFPKPSE EKIKLILNQS 420
AWTSQSNSLA SCLSRLSGKS ETGKTGLINL GNTCYMNSVI QALFMATDFR RQVLSLNLNG 480
CNSLMKKLQH LFAFLAHTQR EAYAPRIFFE ASRPPWFTPR SQQDCSEYLR FLLDRLHEEE 540
KILKVQASHK PSEILECSET SLQEVASKAA VLTETPRTSD GEKTLIEKMF GGKLRTHIRC 600
LNCRSTSQKV EAFTDLSLAF CPSSSLENMS VQDPASSPSI QDGGLMQASV PGPSEEPVVY 660
NPTTAAFICD SLVNEKTIGS PPNEFYCSEN TSVPNESNKI LVNKDVPQKP GGETTPSVTD 720
LLNYFLAPEI LTGDNQYYCE NCASLQNAEK TMQITEEPEY LILTLLRFSY DQKYHVRRKI 780
LDNVSLPLVL ELPVKRITSF SSLSESWSVD VDFTDLSENL AKKLKPSGTD EASCTKLVPY 840
LLSSVVVHSG ISSESGHYYS YARNITSTDS SYQMYHQSEA LALASSQSHL LGRDSPSAVF 900
EQDLENKEMS KEWFLFNDSR VTFTSFQSVQ KITSRFPKDT AYVLLYKKQH STNGLSGNNP 960
TSGLWINGDP PLQKELMDAI TKDNKLYLQE QELNARARAL QAASASCSFR PNGFDDNDPP 1020
GSCGPTGGGG GGGFNTVGRL VF 1042 
Gene Ontology
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF00443; UCH 
SMART
  
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS