CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032151
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA mismatch repair protein Mlh1 
Protein Synonyms/Alias
 cDNA FLJ55289, highly similar to DNA mismatch repair protein Mlh1 
Gene Name
 MLH1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
92VQQHIESKLLGSNSSubiquitination[1, 2, 3]
136STSGSSDKVYAHQMVubiquitination[2]
161AFLQPLSKPLSSQPQacetylation[4]
202APAEVAAKNQSLEGDacetylation[4]
212SLEGDTTKGTSEMSEubiquitination[2]
220GTSEMSEKRGPTSSNacetylation[3]
247MVEDDSRKEMTAACTubiquitination[2]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 460 AA 
Protein Sequence
MNGYISNANY SVKKCIFLLF INHRLVESTS LRKAIETVYA AYLPKNTHPF LYLSLEISPQ 60
NVDVNVHPTK HEVHFLHEES ILERVQQHIE SKLLGSNSSR MYFTQTLLPG LAGPSGEMVK 120
STTSLTSSST SGSSDKVYAH QMVRTDSREQ KLDAFLQPLS KPLSSQPQAI VTEDKTDISS 180
GRARQQDEEM LELPAPAEVA AKNQSLEGDT TKGTSEMSEK RGPTSSNPRK RHREDSDVEM 240
VEDDSRKEMT AACTPRRRII NLTSVLSLQE EINEQGHEVL REMLHNHSFV GCVNPQWALA 300
QHQTKLYLLN TTKLSEELFY QILIYDFANF GVLRLSEPAP LFDLAMLALD SPESGWTEED 360
GPKEGLAEYI VEFLKKKAEM LADYFSLEID EEGNLIGLPL LIDNYVPPLE GLPIFILRLA 420
TEVNWDEEKE CFESLSKECA MFYSIRKQYI SEESTLSGQQ 460 
Gene Ontology
 GO:0005712; C:chiasma; IEA:Compara.
 GO:0001673; C:male germ cell nucleus; IEA:Compara.
 GO:0032389; C:MutLalpha complex; IEA:Compara.
 GO:0000795; C:synaptonemal complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0032137; F:guanine/thymine mispair binding; IEA:Compara.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:Compara.
 GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IEA:Compara.
 GO:0045190; P:isotype switching; IEA:Compara.
 GO:0007060; P:male meiosis chromosome segregation; IEA:Compara.
 GO:0043060; P:meiotic metaphase I plate congression; IEA:Compara.
 GO:0006298; P:mismatch repair; IEA:Compara.
 GO:0045950; P:negative regulation of mitotic recombination; IEA:Compara.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IEA:Compara.
 GO:0048477; P:oogenesis; IEA:Compara.
 GO:0000712; P:resolution of meiotic recombination intermediates; IEA:Compara.
 GO:0016446; P:somatic hypermutation of immunoglobulin genes; IEA:Compara.
 GO:0007283; P:spermatogenesis; IEA:Compara.
 GO:0051257; P:spindle midzone assembly involved in meiosis; IEA:Compara. 
Interpro
 IPR002099; DNA_mismatch_repair.
 IPR013507; DNA_mismatch_repair_C.
 IPR011186; DNA_mismatch_repair_MLH1.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr. 
Pfam
 PF01119; DNA_mis_repair 
SMART
  
PROSITE
  
PRINTS