| Tag | Content |
|---|
| CPLM ID | CPLM-017890 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3 |
Protein Synonyms/Alias | Centaurin-delta-3; Cnt-d3; Dual specificity Rho- and Arf-GTPase-activating protein 1 |
Gene Name | Arap3 |
Gene Synonyms/Alias | Centd3; Drag1; Kiaa4097 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 342 | TRSSKDNKFQVITGQ | ubiquitination | [1] | | 535 | ALGSGISKVQSLKLD | ubiquitination | [1] | | 594 | EFISRKYKLGLFRKP | ubiquitination | [1] | | 1341 | SSDLARQKFGTMPLL | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Acts preferentially on ARF5 and on RHOA. |
Sequence Annotation | DOMAIN 4 68 SAM.
DOMAIN 282 374 PH 1.
DOMAIN 389 478 PH 2.
DOMAIN 479 606 Arf-GAP.
DOMAIN 671 785 PH 3.
DOMAIN 795 901 PH 4.
DOMAIN 903 1084 Rho-GAP.
DOMAIN 1113 1206 Ras-associating.
DOMAIN 1219 1321 PH 5.
MOD_RES 1399 1399 Phosphotyrosine.
MOD_RES 1404 1404 Phosphotyrosine.
MOD_RES 1438 1438 Phosphoserine (By similarity).
MOD_RES 1474 1474 Phosphoserine (By similarity). |
Keyword | Alternative splicing; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; GTPase activation; Membrane; Phosphoprotein; Reference proteome; Repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1538 AA |
Protein Sequence | MAAPQDLDIA VWLALVHLEQ YADTFRRHGL ATAGAAQHLG HEELRHLGIS ATGHRKRILR 60
LLRAGSAEGF LDSHLDNTME PTPSPAPDAQ PPKPVPKPRT VFGLSNPATA QRPGLSPIFW 120
DPEVSRNSEC TQRSSPLLPS SSEQPSVPNT MEMMPNAIYF GLDLRGRAQA AQDVTPDSSQ 180
ATVPTPAFRP TTGTVHIMDP GCLYYGVQPV GIPGASDRRD GRGVCQERAE HRQDLETRED 240
AGYASLELPG DSILSLPTQD AETSDDLISP YASFSSTADR PVPLLSGWLD KLSPQGNYVF 300
QRRFVQFNGR SLMYFGSDKD PFPKGVIPLT AIEMTRSSKD NKFQVITGQR VFVFRTESEA 360
QRDLWCSTLQ SCLKEQRLLG HPRPPHPPRP LRTGTLELRG HKAKVFAALI PGELALYKSE 420
QAFSLGIGIC FIELQGCSVR ETKSRSFDLL TPHRCFSFTA ESGGARQSWA AALQEAVTET 480
LSDYEVAEKV WSNPANRHCA DCRASRPDWA AVNLGVVICK QCAGQHRALG SGISKVQSLK 540
LDTSVWSNEI VQLFIVLGND RANCFWAGAL PPGEGLHPDS APGPRGEFIS RKYKLGLFRK 600
PHPRHPDHSQ LLQALCAAMA GPNLLKNMAQ LLCVETSEGE EPLSPSALNG SLLSLLPSDS 660
PGVYNEVVVP ATYRGFLYCG SISNKAGAPP LRRGRDAPPR LWCVLGAALE MFASESSPEP 720
LSLLQPQDIV CLGVSPPPAD PGDLDRFPFS FELILTGGRI QHFATDGADS LEAWISAVGK 780
WFSPLSCHQL LGPGLLRMGR LWLRSPSHAG LAPGLWLSGF GLLRGDHLFL CPAPGPGPPA 840
PEDMVHLRRL QEISVVSAAD TPDKKEHLVL VETGRTLYLQ GEGRLDFAAW NTAIGGAAGG 900
GGTGLQEQQM SRGDIPIIVD ACISFVTQHG LRLEGVYRKG GARARSLRLL AEFRRDARSV 960
KLRPREHFVE DVTDTLKRFF RELDDPVTSA RLLPRWREAA ELSQKNQRLE KYKEVISCLP 1020
RVNRRTLATL IGHLYRVQKC ASLNQMCTRN LALLFAPSVF QTDGRGEHEV RVLQELIDGY 1080
ISVFDIDSDQ AAQIDLEVSL ITTWKDVQLS QAGDLIMEVY IEQQLPDNCV TLKVSPTLTA 1140
EELTNQVLEM RGAASGTDLW VTFEILEHGE LERPLHPKEK VLEQALQWCQ LPEPCSASLL 1200
LRKVSMAHAG CLFTGVRRES PRVGLLRCRE EPPRLLGNRF QERFFLVRGR CLLLLKEKKS 1260
SKPEREWSLE GAKVYLGIRK KLKPPTLWGF TLILEKMHLC LSCMDEEEMW DWTTSILKAQ 1320
HDDQQSVVLR RRSSSDLARQ KFGTMPLLPI RGDDSGATLL SANQTLRRLH NRRTLSMFFP 1380
MKSPQGSVEE QDELEEPVYE EPVYEEVGAF PELTKDTTFS STWEWSAKSD PSLTSQRSFD 1440
QPPLSKASML GHEERIPDPP PGPPSKSSSQ ARGSLEEQLL QELNNLILRK GEPASCPESS 1500
SQPTSPQAPS PTSLPTPTPS LPTQPPCTSN PPSSQPLT 1538 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:MGI. GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell. GO:0030027; C:lamellipodium; IDA:MGI. GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. GO:0001726; C:ruffle; IDA:MGI. GO:0008060; F:ARF GTPase activator activity; IDA:MGI. GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; IEA:Compara. GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; IEA:Compara. GO:0005100; F:Rho GTPase activator activity; IDA:MGI. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0030336; P:negative regulation of cell migration; IDA:MGI. GO:0035021; P:negative regulation of Rac protein signal transduction; IDA:MGI. GO:0035024; P:negative regulation of Rho protein signal transduction; IDA:MGI. GO:0043547; P:positive regulation of GTPase activity; IEA:GOC. GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro. GO:0008360; P:regulation of cell shape; IDA:MGI. GO:0007165; P:signal transduction; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |