CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013492
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable ATP-dependent RNA helicase DDX46 
Protein Synonyms/Alias
 DEAD box protein 46 
Gene Name
 Ddx46 
Gene Synonyms/Alias
 Kiaa0801 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
895LAPTVSAKTIAEQLAubiquitination[1]
904IAEQLAEKINAKLNYubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Plays an essential role in splicing, either prior to, or during A complex formation (By similarity). 
Sequence Annotation
 DOMAIN 403 581 Helicase ATP-binding.
 DOMAIN 592 753 Helicase C-terminal.
 NP_BIND 416 423 ATP (By similarity).
 MOTIF 372 400 Q motif.
 MOTIF 529 532 DEAD box.
 MOD_RES 263 263 N6-acetyllysine (By similarity).
 MOD_RES 294 294 Phosphotyrosine (By similarity).
 MOD_RES 295 295 Phosphoserine (By similarity).
 MOD_RES 296 296 Phosphoserine (By similarity).
 MOD_RES 776 776 N6-acetyllysine (By similarity).
 MOD_RES 804 804 Phosphoserine.
 MOD_RES 929 929 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Helicase; Hydrolase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1032 AA 
Protein Sequence
MGRESRHYRK RSASRGRSGS RSRSRSPSDK RSKRGDDRRS RSRDRDRRRE RSRSRDKRRS 60
RSRDRKRLRR SRSRERDRSR ERRRSRSRDR RRSRSRSRGR RSRSSSPGSK TKKTENRSRS 120
KEKAEGGDSS KEKKKDKDDK EDEKEKDAGN FDQNKLEEEM RKRKERVEKW REEQRKKAME 180
NIGELKKEIE EMKQGKKWSL EDDDDDEDDP AEAEKEGTEM EDEELDPLDA YMEEVKEEVK 240
KFNMRSVKGG AGNEKKSGPT VTKVVTVVTT KKAVVDADKK KGELMENDQD AMEYSSEEEE 300
VDLQTALTGY QTKQRKLLEP VDHGKIEYEP FRKNFYVEVP ELAKMSQEEV NVFRLEMEGI 360
TVKGKGCPKP IKSWVQCGIS MKILNSLKKH GYEKPTPIQT QAIPAIMSGR DLIGIAKTGS 420
GKTIAFLLPM FRHIMDQRSL EEGEGPIAVI MTPTRELALQ ITKECKKFSK TLGLRVVCVY 480
GGTGISEQIA ELKRGAEIIV CTPGRMIDML AANSGRVTNL RRVTYVVLDE ADRMFDMGFE 540
PQVMRIVDNV RPDRQTVMFS ATFPRAMEAL ARRILSKPIE VQVGGRSVVC SDVEQQVIVI 600
EEEKKFLKLL ELLGHYQESG SVIIFVDKQE HADGLLKDLM RASYPCMSLH GGIDQYDRDS 660
IINDFKNGTC KLLVATSVAA RGLDVKHLIL VVNYSCPNHY EDYVHRAGRT GRAGNKGYAY 720
TFITEDQARY AGDIIKALEL SGTAVPPDLE KLWSDFKDQQ KAEGKIIKKS SGFSGKGFKF 780
DETEQALANE RKKLQKAALG LQDSDDEDAA VDIDEQIESM FNSKKRVKDM AAPGTSSVPA 840
PTAGNAEKLE IAKRLALRIN AQKNLGIESQ VDVMQQATNA ILRGGTILAP TVSAKTIAEQ 900
LAEKINAKLN YVPLEKQEEE RQEGGQSESF KRYEEELEIN DFPQTARWKV TSKEALQRIS 960
EYSEAAITIR GTYFPPGKEP KEGERKIYLA IESANELAVQ KAKAEITRLI KEELIRLQNS 1020
YQPTNKGRYK VL 1032 
Gene Ontology
 GO:0015030; C:Cajal body; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS