CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008719
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coatomer subunit alpha 
Protein Synonyms/Alias
 Alpha-coat protein; Alpha-COP; HEP-COP; HEPCOP; Xenin; Xenopsin-related peptide; Proxenin 
Gene Name
 COPA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61VRGIDFHKQQPLFVSubiquitination[1, 2]
81KIKVWNYKLRRCLFTubiquitination[1, 2]
170DISGLRKKNLSPGAVubiquitination[1, 2]
238IWRMNESKAWEVDTCubiquitination[1, 2, 3]
271ILSNSEDKSIRVWDMubiquitination[1, 2]
350QLDFNSSKDVAVMQLubiquitination[3]
398YDLYTIPKDADSQNPubiquitination[3]
411NPDAPEGKRSSGLTAubiquitination[3]
438RMHSLLIKNLKNEITubiquitination[1]
441SLLIKNLKNEITKKVubiquitination[3]
480TLFDVQQKRTLASVKubiquitination[2, 3]
607TIDPTEFKFKLALINubiquitination[1, 2, 3]
609DPTEFKFKLALINRKubiquitination[1, 2]
628LHMVRNAKLVGQSIIubiquitination[3]
640SIIAYLQKKGYPEVAubiquitination[2, 3]
736MKIAEIRKDMSGHYQubiquitination[1, 2]
834FEGTIASKGKGGALAubiquitination[2, 3]
1037KFEEAVEKFRSILLSubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). 
Sequence Annotation
 REPEAT 7 37 WD 1.
 REPEAT 49 79 WD 2.
 REPEAT 91 121 WD 3.
 REPEAT 133 163 WD 4.
 REPEAT 203 233 WD 5.
 REPEAT 247 277 WD 6.
 MOD_RES 173 173 Phosphoserine.
 MOD_RES 185 185 Phosphothreonine.
 MOD_RES 402 402 Phosphoserine.
 MOD_RES 895 895 Phosphoserine.
 MOD_RES 1035 1035 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; ER-Golgi transport; Golgi apparatus; Hormone; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; RNA editing; Secreted; Transport; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1224 AA 
Protein Sequence
MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH DGPVRGIDFH 60
KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF FHHEYPWILS ASDDQTIRVW 120
NWQSRTCVCV LTGHNHYVMC AQFHPTEDLV VSASLDQTVR VWDISGLRKK NLSPGAVESD 180
VRGITGVDLF GTTDAVVKHV LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW 240
EVDTCRGHYN NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH 300
PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK DVAVMQLRSG 360
SKFPVFNMSY NPAENAVLLC TRASNLENST YDLYTIPKDA DSQNPDAPEG KRSSGLTAVW 420
VARNRFAVLD RMHSLLIKNL KNEITKKVQV PNCDEIFYAG TGNLLLRDAD SITLFDVQQK 480
RTLASVKISK VKYVIWSADM SHVALLAKHE HSCPLPLTAI VICNRKLDAL CNIHENIRVK 540
SGAWDESGVF IYTTSNHIKY AVTTGDHGII RTLDLPIYVT RVKGNNVYCL DRECRPRVLT 600
IDPTEFKFKL ALINRKYDEV LHMVRNAKLV GQSIIAYLQK KGYPEVALHF VKDEKTRFSL 660
ALECGNIEIA LEAAKALDDK NCWEKLGEVA LLQGNHQIVE MCYQRTKNFD KLSFLYLITG 720
NLEKLRKMMK IAEIRKDMSG HYQNALYLGD VSERVRILKN CGQKSLAYLT AATHGLDEEA 780
ESLKETFDPE KETIPDIDPN AKLLQPPAPI MPLDTNWPLL TVSKGFFEGT IASKGKGGAL 840
AADIDIDTVG TEGWGEDAEL QLDEDGFVEA TEGLGDDALG KGQEEGGGWD VEEDLELPPE 900
LDISPGAAGG AEDGFFVPPT KGTSPTQIWC NNSQLPVDHI LAGSFETAMR LLHDQVGVIQ 960
FGPYKQLFLQ TYARGRTTYQ ALPCLPSMYG YPNRNWKDAG LKNGVPAVGL KLNDLIQRLQ 1020
LCYQLTTVGK FEEAVEKFRS ILLSVPLLVV DNKQEIAEAQ QLITICREYI VGLSVETERK 1080
KLPKETLEQQ KRICEMAAYF THSNLQPVHM ILVLRTALNL FFKLKNFKTA ATFARRLLEL 1140
GPKPEVAQQT RKILSACEKN PTDAYQLNYD MHNPFDICAA SYRPIYRGKP VEKCPLSGAC 1200
YSPEFKGQIC RVTTVTEIGK DVIGLRISPL QFR 1233 
Gene Ontology
 GO:0030126; C:COPI vesicle coat; ISS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005615; C:extracellular space; IDA:MGI.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0048205; P:COPI coating of Golgi vesicle; TAS:Reactome.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0030157; P:pancreatic juice secretion; IDA:MGI.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; TAS:Reactome. 
Interpro
 IPR016391; Coatomer_asu.
 IPR010714; Coatomer_asu_C.
 IPR006692; Coatomer_WD-assoc_reg.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04053; Coatomer_WDAD
 PF06957; COPI_C
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.