UniProt Accession

 CH60_ECOLI; P0A6F5; P06139; Q2M6G1 

Genbank Protein ID

 X07850; U14003; U00096; AP009048; X07899; M11294 

Genbank Nucleotide ID

 CAA30698.1; AAA97042.1; AAC77103.1; BAE78145.1; CAA30739.1; AAA23934.1 

Protein Name

 60 kDa chaperonin 

Protein Synonyms/Alias

 GroEL protein; Protein Cpn60 

Gene Name

 groL 

Gene Synonyms/Alias

 groEL; mopA; b4143; JW4103 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
7	*MAAKDVKFGNDARV	acetylation	[1]
28	NVLADAVKVTLGPKG	acetylation	[1]
42	GRNVVLDKSFGAPTI	acetylation	[1, 2, 3]
51	FGAPTITKDGVSVAR	acetylation	[1, 3]
65	REIELEDKFENMGAQ	acetylation	[1]
65	REIELEDKFENMGAQ	pupylation	[4]
80	MVKEVASKANDAAGD	acetylation	[1]
105	AIITEGLKAVAAGMN	acetylation	[1, 3]
117	GMNPMDLKRGIDKAV	acetylation	[1, 3, 5]
122	DLKRGIDKAVTAAVE	acetylation	[1, 2, 3]
132	TAAVEELKALSVPCS	acetylation	[1, 3]
142	SVPCSDSKAIAQVGT	acetylation	[1, 3]
160	NSDETVGKLIAEAMD	acetylation	[1, 3]
168	LIAEAMDKVGKEGVI	acetylation	[1, 3]
168	LIAEAMDKVGKEGVI	pupylation	[4]
207	LSPYFINKPETGAVE	acetylation	[1, 3]
225	PFILLADKKISNIRE	acetylation	[1]
226	FILLADKKISNIREM	acetylation	[3]
242	PVLEAVAKAGKPLLI	acetylation	[1, 3]
272	NTMRGIVKVAAVKAP	acetylation	[1, 3]
277	IVKVAAVKAPGFGDR	acetylation	[1, 3]
321	LEDLGQAKRVVINKD	acetylation	[1, 3]
321	LEDLGQAKRVVINKD	pupylation	[4]
327	AKRVVINKDTTTIID	acetylation	[1, 3]
364	TSDYDREKLQERVAK	acetylation	[1, 3]
371	KLQERVAKLAGGVAV	acetylation	[1]
390	AATEVEMKEKKARVE	acetylation	[1]
425	ALIRVASKLADLRGQ	acetylation	[1, 2, 3]
441	EDQNVGIKVALRAME	acetylation	[1, 3]
498	MGILDPTKVTRSALQ	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [4] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222]
 [5] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

 Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. 

Sequence Annotation

 MOD_RES 34 34 N6-succinyllysine.
 MOD_RES 51 51 N6-succinyllysine.
 MOD_RES 117 117 N6-acetyllysine; alternate.
 MOD_RES 117 117 N6-succinyllysine; alternate.
 MOD_RES 277 277 N6-succinyllysine.
 MOD_RES 321 321 N6-succinyllysine.
 MOD_RES 390 390 N6-succinyllysine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Cell cycle; Cell division; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 548 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:EcoCyc.
 GO:0016887; F:ATPase activity; IDA:EcoCyc.
 GO:0051082; F:unfolded protein binding; IMP:EcoCyc.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0006457; P:protein folding; IMP:EcoCyc.
 GO:0042026; P:protein refolding; IEA:HAMAP.
 GO:0009408; P:response to heat; IEP:EcoliWiki.
 GO:0019068; P:virion assembly; IMP:EcoliWiki. 

Interpro

 IPR018370; Chaperonin_Cpn60_CS.
 IPR001844; Chaprnin_Cpn60.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial. 

Pfam

 PF00118; Cpn60_TCP1 

SMART

  

PROSITE

 PS00296; CHAPERONINS_CPN60 

PRINTS

 PR00298; CHAPERONIN60.