UniProt Accession

 M2GD_MOUSE; Q9DBT9; B1B1D0; Q8R1S7 

Genbank Protein ID

 AK004755; CT030023; AC131739; BC024126 

Genbank Nucleotide ID

 BAB23536.1; CAO77970.1; CAO77970.1; AAH24126.1 

Protein Name

 Dimethylglycine dehydrogenase, mitochondrial 

Protein Synonyms/Alias

 ME2GLYDH 

Gene Name

 Dmgdh 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
40	PASPPEWKDRAETVI	acetylation	[1, 2, 3, 4]
40	PASPPEWKDRAETVI	succinylation	[3]
107	KIHYDSIKLYERLEE	acetylation	[1, 2, 4, 5, 6, 7]
141	PVRVDEFKYQMTRTN	acetylation	[1, 2, 3, 4, 7]
141	PVRVDEFKYQMTRTN	succinylation	[3]
161	QYIIEPEKIHELFPL	acetylation	[2, 4, 7]
173	FPLLNMNKILAGLYN	acetylation	[4]
201	ALAAGARKYGALLKY	acetylation	[7]
201	ALAAGARKYGALLKY	ubiquitination	[8]
207	RKYGALLKYPAPVTS	acetylation	[2, 3]
207	RKYGALLKYPAPVTS	succinylation	[3]
216	PAPVTSLKPRPDGTW	acetylation	[2, 3, 4, 7]
216	PAPVTSLKPRPDGTW	succinylation	[3]
250	FWAREVGKMIGLDHP	acetylation	[2, 3, 7]
250	FWAREVGKMIGLDHP	succinylation	[3]
275	TSTIPEVKALKRELP	acetylation	[4, 7]
310	GPYESQEKMKLQASW	acetylation	[1, 2, 3, 4, 7]
310	GPYESQEKMKLQASW	succinylation	[3]
310	GPYESQEKMKLQASW	ubiquitination	[8]
312	YESQEKMKLQASWVT	acetylation	[3, 7]
312	YESQEKMKLQASWVT	succinylation	[3]
328	GVPPGFGKELFESDL	acetylation	[3, 4, 7]
328	GVPPGFGKELFESDL	succinylation	[3]
353	MEMIPVLKKADIINV	acetylation	[4, 7]
427	LDPNRYGKWTTTQYT	acetylation	[1, 2, 3, 4, 7]
427	LDPNRYGKWTTTQYT	succinylation	[3]
427	LDPNRYGKWTTTQYT	ubiquitination	[8]
437	TTQYTEAKARESYGF	ubiquitination	[8]
469	QRVSGLYKTLKSKCS	acetylation	[2, 3, 4, 7]
469	QRVSGLYKTLKSKCS	succinylation	[3]
469	QRVSGLYKTLKSKCS	ubiquitination	[8]
472	SGLYKTLKSKCSMGF	acetylation	[2]
491	EQPHWFYKPGQDTQY	acetylation	[4]
516	EPVGSEYKQVMQRVG	acetylation	[1, 2, 3, 4, 6, 7]
516	EPVGSEYKQVMQRVG	succinylation	[3]
532	IDLSPFGKFNIKGRD	ubiquitination	[8]
636	YARRVLQKLTSEDLS	ubiquitination	[8]
648	DLSDDAFKFLQTKSF	acetylation	[1, 2, 3, 4, 7]
648	DLSDDAFKFLQTKSF	succinylation	[3]
653	AFKFLQTKSFNISDI	acetylation	[2, 4, 7]
653	AFKFLQTKSFNISDI	ubiquitination	[8]
743	EYFVKLNKPADFIGK	ubiquitination	[8]
750	KPADFIGKQALKQIK	acetylation	[2]
754	FIGKQALKQIKTEGL	acetylation	[2, 4, 7]
757	KQALKQIKTEGLKRR	acetylation	[2, 4, 7]
762	QIKTEGLKRRLVCLT	acetylation	[2]
786	GNESIWYKGKVVGNT	acetylation	[1, 3, 4, 5, 6, 7]
786	GNESIWYKGKVVGNT	succinylation	[3]
788	ESIWYKGKVVGNTTS	acetylation	[2, 3]
788	ESIWYKGKVVGNTTS	succinylation	[3]
788	ESIWYKGKVVGNTTS	ubiquitination	[8]
852	PARARLQKDGKKTNL	acetylation	[2]
861	GKKTNLEKGPSRTTK	acetylation	[2, 7]

Reference

 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [7] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [8] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

  

Sequence Annotation

 MOD_RES 84 84 Tele-8alpha-FAD histidine (By
 MOD_RES 866 866 Phosphothreonine.  

Keyword

 Complete proteome; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 869 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004047; F:aminomethyltransferase activity; IEA:InterPro.
 GO:0047865; F:dimethylglycine dehydrogenase activity; IEA:EC.
 GO:0006579; P:amino-acid betaine catabolic process; IEA:UniProtKB-UniPathway.
 GO:0006546; P:glycine catabolic process; IEA:InterPro. 

Interpro

 IPR006076; FAD-dep_OxRdtase.
 IPR013977; GCV_T_C.
 IPR006222; GCV_T_N.
 IPR027266; TrmE/GcvT_dom1. 

Pfam

 PF01266; DAO
 PF01571; GCV_T
 PF08669; GCV_T_C 

SMART

  

PROSITE

  

PRINTS