CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019750
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Prohibitin-2 
Protein Synonyms/Alias
 B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity 
Gene Name
 PHB2 
Gene Synonyms/Alias
 BAP; REA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MAQNLKDLAGRLPubiquitination[1, 2, 3, 4]
89DIRARPRKISSPTGSubiquitination[2]
97ISSPTGSKDLQMVNIubiquitination[1, 2, 4, 5]
142SIVNEVLKSVVAKFNubiquitination[3, 5]
147VLKSVVAKFNASQLIubiquitination[1, 4, 5]
200YTAAVEAKQVAQQEAubiquitination[2, 5, 6]
216RAQFLVEKAKQEQRQubiquitination[2, 5]
218QFLVEKAKQEQRQKIubiquitination[3]
224AKQEQRQKIVQAEGEubiquitination[2, 3]
236EGEAEAAKMLGEALSubiquitination[2, 3, 5]
244MLGEALSKNPGYIKLubiquitination[1, 2, 3, 4, 5, 7]
250SKNPGYIKLRKIRAAacetylation[8]
250SKNPGYIKLRKIRAAubiquitination[2, 3, 5]
262RAAQNISKTIATSQNubiquitination[2, 3, 5, 7]
296RGSDSLIKGKK****ubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. 
Sequence Annotation
 REGION 19 49 Necessary for transcriptional repression.
 REGION 150 174 Necessary for transcriptional repression.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 128 128 Phosphotyrosine.
 MOD_RES 250 250 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleus; Phosphoprotein; Receptor; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 299 AA 
Protein Sequence
MAQNLKDLAG RLPAGPRGMG TALKLLLGAG AVAYGVRESV FTVEGGHRAI FFNRIGGVQQ 60
DTILAEGLHF RIPWFQYPII YDIRARPRKI SSPTGSKDLQ MVNISLRVLS RPNAQELPSM 120
YQRLGLDYEE RVLPSIVNEV LKSVVAKFNA SQLITQRAQV SLLIRRELTE RAKDFSLILD 180
DVAITELSFS REYTAAVEAK QVAQQEAQRA QFLVEKAKQE QRQKIVQAEG EAEAAKMLGE 240
ALSKNPGYIK LRKIRAAQNI SKTIATSQNR IYLTADNLVL NLQDESFTRG SDSLIKGKK 299 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0030331; F:estrogen receptor binding; NAS:UniProtKB.
 GO:0060749; P:mammary gland alveolus development; IEA:Compara.
 GO:0060744; P:mammary gland branching involved in thelarche; IEA:Compara.
 GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; IEA:Compara.
 GO:0033600; P:negative regulation of mammary gland epithelial cell proliferation; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0060762; P:regulation of branching involved in mammary gland duct morphogenesis; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001107; Band_7.
 IPR000163; Prohibitin. 
Pfam
 PF01145; Band_7 
SMART
 SM00244; PHB 
PROSITE
  
PRINTS
 PR00679; PROHIBITIN.