CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019370
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1,5-anhydro-D-fructose reductase 
Protein Synonyms/Alias
 AF reductase; Aldo-keto reductase family 1 member C-like protein 2; Aldo-keto reductase family 1 member E2; LoopADR; Testis-specific protein; hTSP 
Gene Name
 AKR1E2 
Gene Synonyms/Alias
 AKR1CL2; AKRDC1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13AVGLSSWKASPGKVTubiquitination[1]
183QLERLLNKPGLRFKPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the NADPH-dependent reduction of 1,5-anhydro- D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones (By similarity). Has low NADPH-dependent reductase activity towards 9,10- phenanthrenequinone (in vitro). 
Sequence Annotation
 NP_BIND 269 277 NADP (By similarity).
 ACT_SITE 40 40 Proton donor (By similarity).
 BINDING 35 35 NADP (By similarity).
 BINDING 102 102 Substrate (By similarity).
 BINDING 193 193 NADP (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; NADP; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 320 AA 
Protein Sequence
MGDIPAVGLS SWKASPGKVT EAVKEAIDAG YRHFDCAYFY HNEREVGAGI RCKIKEGAVR 60
REDLFIATKL WCTCHKKSLV ETACRKSLKA LKLNYLDLYL IHWPMGFKPP HPEWIMSCSE 120
LSFCLSHPRV QDLPLDESNM VIPSDTDFLD TWEAMEDLVI TGLVKNIGVS NFNHEQLERL 180
LNKPGLRFKP LTNQIECHPY LTQKNLISFC QSRDVSVTAY RPLGGSCEGV DLIDNPVIKR 240
IAKEHGKSPA QILIRFQIQR NVIVIPGSIT PSHIKENIQV FDFELTQHDM DNILSLNRNL 300
RLAMFPITKN HKDYPFHIEY 320 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0050571; F:1,5-anhydro-D-fructose reductase activity; IEA:EC. 
Interpro
 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 
Pfam
 PF00248; Aldo_ket_red 
SMART
  
PROSITE
 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 
PRINTS
 PR00069; ALDKETRDTASE.