| Tag | Content |
|---|
| CPLM ID | CPLM-006364 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Nuclear cap-binding protein complex subunit 1 |
Protein Synonyms/Alias | 80 kDa nuclear cap-binding protein; CBP80; NCBP 80 kDa subunit; Glycolysis regulation protein 3; Protein SUT1; Suppressor of TOP1 protein |
Gene Name | STO1 |
Gene Synonyms/Alias | CBC1; CBP80; GCR3; SUT1; YMR125W; YM8564.07; YM9553.01 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 509 | DDSIKFGKYFYNPKV | acetylation | [1] | | 520 | NPKVNFAKNLIQKEL | ubiquitination | [2] | | 525 | FAKNLIQKELRLTSN | ubiquitination | [2] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Component of the CBC complex, which binds co- transcriptionally to the 5'-cap of pre-mRNAs and is involved in maturation, export and degradation of nuclear mRNAs. The CBC complex is required for efficient pre-mRNA splicing through efficient commitment complex and spliceosome formation. Together with NPL3, the CBC complex is required for export of mRNAs out of the nucleus. The CBC complex is also involved in nuclear mRNA degradation, probably by directing the mRNAs to the sites of degradation. Affects replication of the positive-strand RNA virus BMV. |
Sequence Annotation | DOMAIN 36 264 MIF4G.
MOTIF 22 30 Nuclear localization signal (Potential). |
Keyword | 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; mRNA capping; mRNA processing; mRNA splicing; mRNA transport; Nonsense-mediated mRNA decay; Nucleus; Reference proteome; RNA-binding; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 861 AA |
Protein Sequence | MFNRKRRGDF DEDENYRDFR PRMPKRQRIP PVVQLCKEMM PDIRTIGESV KAFEDDIKFL 60
SEAIMNEYGH EDYFNNALLS TLNAVVVEQP QKQAAIALLT MVVNSKNNVA GKSIINYFFE 120
ELQKWCKQTY NDEFKSTSNE TGPWNKIKLI LRFLSILSPM FLVDELINIY KSLFELSIEL 180
NNLDPGNRVP LSEAIYTNTL LNIPYLFFFN RNNDGLRTKV EELLAYVEQN YLVKTTDINL 240
LREYNGEPPY EMVELVRVVL PNVKKALINN LEQLNELFPD WNHLLTPQTG DEGFNDALTL 300
PSVDDLKSFV RLNKNFGSVD SMWKTPRYAF HVYLPNSAGN FETVVPISTY AGQLFNDIII 360
DLVESLEFNR KEVARQVITL DLFFKAGIFT EPGESIAQLI ATYEENPLAP TFKIEDLAIE 420
TILGLIFKLP SVSQPFAYFY TLLVDICQNS PKAIAPVFGR AFRFFYSHLD SLDFELKLRY 480
LDWFSIQMSN FNFSWKWNEW EDDSIKFGKY FYNPKVNFAK NLIQKELRLT SNFSEVEDSL 540
PQEFTKYLDT SYIPRDQLIN YYQSLFTGYT VEEDSVRKND LYFRQEGVPM ENTVRKILDY 600
THKANNSREV TELESILGEL KNEYGSIISD FNRFVIILLV QAVTDSGSRS LSHANKYIND 660
LKEDLKTIFA KIELDIETKE YIIIEAVLTF WNANPQTGFL VADAFKYAGL LTSRTIFTFI 720
FNETGLKNNG LIEATAIEAV FRNLSQQISE ENESGNNFEF VFERLCTIAN STIDLLDVNA 780
DEDIEIPKVN GEMDIDDIED DKLDLKWKYF TVIGFIKSIL RRYSHEYREL ADKFIANIDN 840
AIPHESTRRT ISNWIQETKE V 861 |
Gene Ontology | GO:0000243; C:commitment complex; IPI:SGD. GO:0005846; C:nuclear cap binding complex; IDA:SGD. GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell. GO:0005844; C:polysome; IDA:SGD. GO:0003677; F:DNA binding; IEA:InterPro. GO:0003729; F:mRNA binding; IPI:SGD. GO:0000339; F:RNA cap binding; IEA:InterPro. GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW. GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:InterPro. GO:0000398; P:mRNA splicing, via spliceosome; IPI:SGD. GO:0051028; P:mRNA transport; IEA:UniProtKB-KW. GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:SGD. GO:0006970; P:response to osmotic stress; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |