UniProt Accession

 NDUS1_HUMAN; P28331; B4DIN9; B4DJA0; B4DPG1; B4DUC1; E7ENF3; Q53TR8; Q8N1C4; Q8TCC9 

Genbank Protein ID

 X61100; AK295705; AK295987; AK298320; AK300585; AC007383; CH471063; BC022368; BC030833 

Genbank Nucleotide ID

 CAA43412.1; BAG58551.1; BAG58762.1; BAG60573.1; BAG62283.1; AAY15061.1; EAW70379.1; AAH22368.1; AAH30833.1 

Protein Name

 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial 

Protein Synonyms/Alias

 Complex I-75kD; CI-75kD 

Gene Name

 NDUFS1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
108	NILTNSEKSKKAREG	ubiquitination	[1]
289	NEEWISDKTRFAYDG	ubiquitination	[1]
298	RFAYDGLKRQRLTEP	ubiquitination	[1]
311	EPMVRNEKGLLTYTS	ubiquitination	[1]
467	HPFSQVLKEAKKPMV	acetylation	[2]
467	HPFSQVLKEAKKPMV	ubiquitination	[1]
499	AVSSIAQKIRMTSGV	ubiquitination	[1]
531	AALDLGYKPGVEAIR	ubiquitination	[1]
607	EGRAQQTKVAVTPPG	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. 

Sequence Annotation

 DOMAIN 30 108 2Fe-2S ferredoxin-type.
 DOMAIN 245 301 4Fe-4S Mo/W bis-MGD-type.
 METAL 64 64 Iron-sulfur 1 (2Fe-2S) (By similarity).
 METAL 75 75 Iron-sulfur 1 (2Fe-2S) (By similarity).
 METAL 78 78 Iron-sulfur 1 (2Fe-2S) (By similarity).
 METAL 92 92 Iron-sulfur 1 (2Fe-2S) (By similarity).
 METAL 124 124 Iron-sulfur 2 (4Fe-4S); via pros nitrogen
 METAL 128 128 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 131 131 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 137 137 Iron-sulfur 2 (4Fe-4S) (By similarity).
 METAL 176 176 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 179 179 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 182 182 Iron-sulfur 3 (4Fe-4S) (By similarity).
 METAL 226 226 Iron-sulfur 3 (4Fe-4S) (By similarity).
 MOD_RES 84 84 N6-acetyllysine (By similarity).  

Keyword

 2Fe-2S; 4Fe-4S; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Polymorphism; Reference proteome; Respiratory chain; Transit peptide; Transport; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 727 AA 

Protein Sequence

Gene Ontology

 GO:0005758; C:mitochondrial intermembrane space; IDA:UniProtKB.
 GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
 GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0009055; F:electron carrier activity; NAS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; NAS:UniProtKB.
 GO:0008637; P:apoptotic mitochondrial changes; IDA:UniProtKB.
 GO:0046034; P:ATP metabolic process; IMP:UniProtKB.
 GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; NAS:UniProtKB.
 GO:0072593; P:reactive oxygen species metabolic process; IMP:UniProtKB.
 GO:0051881; P:regulation of mitochondrial membrane potential; IMP:UniProtKB. 

Interpro

 IPR001041; 2Fe-2S_ferredoxin-type.
 IPR012675; Beta-grasp_dom.
 IPR006656; Mopterin_OxRdtase.
 IPR000283; NADH_UbQ_OxRdtase_75kDa_su_CS.
 IPR010228; NADH_UbQ_OxRdtase_Gsu.
 IPR019574; NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
 IPR015405; NuoG_C. 

Pfam

 PF09326; DUF1982
 PF00384; Molybdopterin
 PF10588; NADH-G_4Fe-4S_3 

SMART

 SM00929; NADH-G_4Fe-4S_3 

PROSITE

 PS00197; 2FE2S_FER_1
 PS51085; 2FE2S_FER_2
 PS51669; 4FE4S_MOW_BIS_MGD
 PS00641; COMPLEX1_75K_1
 PS00642; COMPLEX1_75K_2
 PS00643; COMPLEX1_75K_3 

PRINTS