CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023194
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Targeting protein for Xklp2 
Protein Synonyms/Alias
 Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Protein fls353; Restricted expression proliferation-associated protein 100; p100 
Gene Name
 TPX2 
Gene Synonyms/Alias
 C20orf1; C20orf2; DIL2; HCA519 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44EKANLENKLLGKNGTubiquitination[1]
48LENKLLGKNGTGGLFubiquitination[1]
75QAIVTPLKPVDNTYYubiquitination[1]
305TKGCTIVKPFNLSQGacetylation[2]
560KKIKELQKGEVPKFKubiquitination[1]
567KGEVPKFKALPLPHFubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. 
Sequence Annotation
 MOD_RES 59 59 Phosphothreonine.
 MOD_RES 72 72 Phosphothreonine.
 MOD_RES 292 292 Phosphoserine.
 MOD_RES 293 293 Phosphoserine.
 MOD_RES 305 305 N6-acetyllysine.
 MOD_RES 310 310 Phosphoserine.
 MOD_RES 338 338 Phosphothreonine.
 MOD_RES 369 369 Phosphothreonine.
 MOD_RES 486 486 Phosphoserine.
 MOD_RES 738 738 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Apoptosis; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 747 AA 
Protein Sequence
MSQVKSSYSY DAPSDFINFS SLDDEGDTQN IDSWFEEKAN LENKLLGKNG TGGLFQGKTP 60
LRKANLQQAI VTPLKPVDNT YYKEAEKENL VEQSIPSNAC SSLEVEAAIS RKTPAQPQRR 120
SLRLSAQKDL EQKEKHHVKM KAKRCATPVI IDEILPSKKM KVSNNKKKPE EEGSAHQDTA 180
EKNASSPEKA KGRHTVPCMP PAKQKFLKST EEQELEKSMK MQQEVVEMRK KNEEFKKLAL 240
AGIGQPVKKS VSQVTKSVDF HFRTDERIKQ HPKNQEEYKE VNFTSELRKH PSSPARVTKG 300
CTIVKPFNLS QGKKRTFDET VSTYVPLAQQ VEDFHKRTPN RYHLRSKKDD INLLPSKSSV 360
TKICRDPQTP VLQTKHRARA VTCKSTAELE AEELEKLQQY KFKARELDPR ILEGGPILPK 420
KPPVKPPTEP IGFDLEIEKR IQERESKKKT EDEHFEFHSR PCPTKILEDV VGVPEKKVLP 480
ITVPKSPAFA LKNRIRMPTK EDEEEDEPVV IKAQPVPHYG VPFKPQIPEA RTVEICPFSF 540
DSRDKERQLQ KEKKIKELQK GEVPKFKALP LPHFDTINLP EKKVKNVTQI EPFCLETDRR 600
GALKAQTWKH QLEEELRQQK EAACFKARPN TVISQEPFVP KKEKKSVAEG LSGSLVQEPF 660
QLATEKRAKE RQELEKRMAE VEAQKAQQLE EARLQEEEQK KEELARLRRE LVHKANPIRK 720
YQGLEIKSSD QPLTVPVSPK FSTRFHC 747 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005819; C:spindle; ISS:UniProtKB.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; TAS:ProtInc.
 GO:0005525; F:GTP binding; TAS:ProtInc.
 GO:0032147; P:activation of protein kinase activity; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0060236; P:regulation of mitotic spindle organization; ISS:UniProtKB. 
Interpro
 IPR015128; Aurora-A-bd.
 IPR027330; TPX2_central_dom.
 IPR027329; TPX2_dom_C.
 IPR009675; TPX2_fam. 
Pfam
 PF09041; Aurora-A_bind
 PF06886; TPX2
 PF12214; TPX2_importin 
SMART
  
PROSITE
  
PRINTS