CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006849
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase alpha subunit B 
Protein Synonyms/Alias
 DNA polymerase I subunit B; DNA polymerase alpha:primase complex p86 subunit; Pol alpha-primase complex p86 subunit; DNA polymerase-primase complex p74 subunit 
Gene Name
 POL12 
Gene Synonyms/Alias
 YBL035C; YBL0414 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
84NQISSSSKVNTSTKKmethylation[1]
Reference
 [1] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074] 
Functional Description
 Non-catalytic component of DNA polymerase alpha, which in a complex with DNA primase (DNA polymerase alpha:primase) constitutes a replicative polymerase. POL12 may play an essential role at the early stage of chromosomal DNA replication by coupling DNA polymerase alpha to the cellular replication machinery (By similarity). Interacts with MCM10. 
Sequence Annotation
 MOD_RES 126 126 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; DNA replication; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 705 AA 
Protein Sequence
MSGSIDVITH FGPDADKPEI ITALENLTKL HALSVEDLYI KWEQFSNQRR QTHTDLTSKN 60
IDEFKQFLQL QMEKRANQIS SSSKVNTSTK KPVIKKSLNS SPLFGLSIPK TPTLKKRKLH 120
GPFSLSDSKQ TYNVGSEAET NEKGNSSLKL EFTPGMAEDA VGDSAPLSHA KSSDAKTPGS 180
STFQTPTTNT PTTSRQNVPA GEILDSLNPE NIEISSGNPN VGLLSTEEPS YNQVKVEPFY 240
DAKKYKFRTM RQNLQEASDV LDDQIESFTK IIQNHYKLSP NDFADPTIQS QSEIYAVGRI 300
VPDSPTYDKF LNPESLSLET SRMGGVGRRV RLDLSQVNEL SFFLGQIVAF KGKNANGDYF 360
TVNSILPLPY PNSPVSTSQE LQEFQANLEG SSLKVIVTCG PYFANDNFSL ELLQEFIDSI 420
NNEVKPHVLI MFGPFIDITH PLIASGKLPN FPQFKTQPKT LDELFLKLFT PILKTISPHI 480
QTVLIPSTKD AISNHAAYPQ ASLIRKALQL PKRNFKCMAN PSSFQINEIY FGCSNVDTFK 540
DLKEVIKGGT TSSRYRLDRV SEHILQQRRY YPIFPGSIRT RIKPKDVSTK KETNDMESKE 600
EKVYEHISGA DLDVSYLGLT EFVGGFSPDI MIIPSELQHF ARVVQNVVVI NPGRFIRATG 660
NRGSYAQITV QCPDLEDGKL TLVEGEEPVY LHNVWKRARV DLIAS 705 
Gene Ontology
 GO:0005658; C:alpha DNA polymerase:primase complex; IDA:SGD.
 GO:0005635; C:nuclear envelope; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
 GO:0006270; P:DNA replication initiation; IMP:SGD.
 GO:0006273; P:lagging strand elongation; IC:SGD.
 GO:0016233; P:telomere capping; IMP:SGD. 
Interpro
 IPR007185; DNA_pol_alpha/epsilon_bsu.
 IPR016722; DNA_pol_alpha_bsu.
 IPR013627; Pol_alpha_B_N. 
Pfam
 PF04042; DNA_pol_E_B
 PF08418; Pol_alpha_B_N 
SMART
  
PROSITE
  
PRINTS