CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001480
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 UFM1-protein ligase 1 
Protein Synonyms/Alias
 LZAP-binding protein 
Gene Name
 UFL1 
Gene Synonyms/Alias
 KIAA0776; NLBP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43VNKLIAQKQLEVVHTubiquitination[1, 2]
571DTQAALTKHLLKSVCacetylation[3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3. 
Sequence Annotation
 REGION 1 212 Required for E3 UFM1-protein ligase
 REGION 121 250 Involved in CDK5RAP3-binding.
 MOD_RES 458 458 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Endoplasmic reticulum; Ligase; Phosphoprotein; Polymorphism; Reference proteome; Tumor suppressor; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 794 AA 
Protein Sequence
MADAWEEIRR LAADFQRAQF AEATQRLSER NCIEIVNKLI AQKQLEVVHT LDGKEYITPA 60
QISKEMRDEL HVRGGRVNIV DLQQVINVDL IHIENRIGDI IKSEKHVQLV LGQLIDENYL 120
DRLAEEVNDK LQESGQVTIS ELCKTYDLPG NFLTQALTQR LGRIISGHID LDNRGVIFTE 180
AFVARHKARI RGLFSAITRP TAVNSLISKY GFQEQLLYSV LEELVNSGRL RGTVVGGRQD 240
KAVFVPDIYS RTQSTWVDSF FRQNGYLEFD ALSRLGIPDA VSYIKKRYKT TQLLFLKAAC 300
VGQGLVDQVE ASVEEAISSG TWVDIAPLLP TSLSVEDAAI LLQQVMRAFS KQASTVVFSD 360
TVVVSEKFIN DCTELFRELM HQKAEKEMKN NPVHLITEED LKQISTLESV STSKKDKKDE 420
RRRKATEGSG SMRGGGGGNA REYKIKKVKK KGRKDDDSDD ESQSSHTGKK KPEISFMFQD 480
EIEDFLRKHI QDAPEEFISE LAEYLIKPLN KTYLEVVRSV FMSSTTSASG TGRKRTIKDL 540
QEEVSNLYNN IRLFEKGMKF FADDTQAALT KHLLKSVCTD ITNLIFNFLA SDLMMAVDDP 600
AAITSEIRKK ILSKLSEETK VALTKLHNSL NEKSIEDFIS CLDSAAEACD IMVKRGDKKR 660
ERQILFQHRQ ALAEQLKVTE DPALILHLTS VLLFQFSTHS MLHAPGRCVP QIIAFLNSKI 720
PEDQHALLVK YQGLVVKQLV SQSKKTGQGD YPLNNELDKE QEDVASTTRK ELQELSSSIK 780
DLVLKSRKSS VTEE 794 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0071568; F:UFM1 conjugating enzyme activity; IDA:UniProtKB.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IMP:UniProtKB.
 GO:0031397; P:negative regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0071569; P:protein ufmylation; IDA:UniProtKB. 
Interpro
 IPR018611; E3_UFM1_ligase_1. 
Pfam
 PF09743; DUF2042 
SMART
  
PROSITE
  
PRINTS