CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020404
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Diphthamide biosynthesis protein 1 
Protein Synonyms/Alias
 DPH1 homolog; HsDph1; Diphthamide biosynthesis protein 2 homolog-like 1; DPH-like 1; DPH2-like 1; Diphthamide biosynthesis protein 2-like; Ovarian cancer-associated gene 1 protein 
Gene Name
 DPH1 
Gene Synonyms/Alias
 DPH2L; DPH2L1; OVCA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43QIPPEILKNPQLQAAubiquitination[1, 2]
64NYNFEIPKTIWRIQQubiquitination[3, 4]
75RIQQAQAKKVALQMPubiquitination[1]
209RVSVPQCKPLSPGEIubiquitination[2, 4]
226CTSPRLSKEVEAVVYubiquitination[1]
261YRYDPYSKVLSREHYubiquitination[1, 2, 3, 4]
288IATARSAKSWGLILGubiquitination[1, 2, 3, 4]
304LGRQGSPKILEHLESubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival (By similarity). 
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Polymorphism; Reference proteome; Tumor suppressor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 443 AA 
Protein Sequence
MRRQVMAALV VSGAAEQGGR DGPGRGRAPR GRVANQIPPE ILKNPQLQAA IRVLPSNYNF 60
EIPKTIWRIQ QAQAKKVALQ MPEGLLLFAC TIVDILERFT EAEVMVMGDV TYGACCVDDF 120
TARALGADFL VHYGHSCLIP MDTSAQDFRV LYVFVDIRID TTHLLDSLRL TFPPATALAL 180
VSTIQFVSTL QAAAQELKAE YRVSVPQCKP LSPGEILGCT SPRLSKEVEA VVYLGDGRFH 240
LESVMIANPN VPAYRYDPYS KVLSREHYDH QRMQAARQEA IATARSAKSW GLILGTLGRQ 300
GSPKILEHLE SRLRALGLSF VRLLLSEIFP SKLSLLPEVD VWVQVACPRL SIDWGTAFPK 360
PLLTPYEAAV ALRDISWQQP YPMDFYAGSS LGPWTVNHGQ DRRPHAPGRP ARGKVQEGSA 420
RPPSAVACED CSCRDEKVAP LAP 443 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IEA:InterPro.
 GO:0006412; P:translation; TAS:ProtInc. 
Interpro
 IPR002728; DPH1/DPH2.
 IPR016435; eu_DPH1/arc_DPH2. 
Pfam
 PF01866; Diphthamide_syn 
SMART
  
PROSITE
  
PRINTS