CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007534
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histone-lysine N-methyltransferase E(z) 
Protein Synonyms/Alias
 Lysine N-methyltransferase 6; Protein enhancer of zeste 
Gene Name
 E(z) 
Gene Synonyms/Alias
 KMT6; CG6502 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
20RVKSEYIKIRQQKRYacetylation[1]
727HRIGIFAKRAIQPGEacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 Polycomb group (PcG) protein. Catalytic subunit of the Esc/E(z) complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required. 
Sequence Annotation
 DOMAIN 443 491 SANT.
 DOMAIN 518 619 CXC.
 DOMAIN 626 741 SET.
 MOTIF 505 510 Nuclear localization signal (Potential).
 MOD_RES 493 493 Phosphoserine.
 MOD_RES 502 502 Phosphothreonine.  
Keyword
 Chromatin regulator; Complete proteome; Developmental protein; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Repressor; S-adenosyl-L-methionine; Transcription; Transcription regulation; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 760 AA 
Protein Sequence
MNSTKVPPEW KRRVKSEYIK IRQQKRYKRA DEIKEAWIRN WDEHNHNVQD LYCESKVWQA 60
KPYDPPHVDC VKRAEVTSYN GIPSGPQKVP ICVINAVTPI PTMYTWAPTQ QNFMVEDETV 120
LHNIPYMGDE VLDKDGKFIE ELIKNYDGKV HGDKDPSFMD DAIFVELVHA LMRSYSKELE 180
EAAPGTATAI KTETLAKSKQ GEDDGVVDVD ADGESPMKLE KTDSKGDLTE VEKKETEEPL 240
ETEDADVKPD VEEVKDKLPF PAPIIFQAIS ANFPDKGTAQ ELKEKYIELT EHQDPERPQE 300
CTPNIDGIKA ESVSRERTMH SFHTLFCRRC FKYDCFLHRL QGHAGPNLQK RRYPELKPFA 360
EPCSNSCYML IDGMKEKLAA DSKTPPIDSC NEASSEDSND SNSQFSNKDF NHENSKDNGL 420
TVNSAAVAEI NSIMAGMMNI TSTQCVWTGA DQALYRVLHK VYLKNYCAIA HNMLTKTCRQ 480
VYEFAQKEDA EFSFEDLRQD FTPPRKKKKK QRLWSLHCRK IQLKKDSSSN HVYNYTPCDH 540
PGHPCDMNCS CIQTQNFCEK FCNCSSDCQN RFPGCRCKAQ CNTKQCPCYL AVRECDPDLC 600
QACGADQFKL TKITCKNVCV QRGLHKHLLM APSDIAGWGI FLKEGAQKNE FISEYCGEII 660
SQDEADRRGK VYDKYMCSFL FNLNNDFVVD ATRKGNKIRF ANHSINPNCY AKVMMVTGDH 720
RIGIFAKRAI QPGEELFFDY RYGPTEQLKF VGIEREMEIV 760 
Gene Ontology
 GO:0035098; C:ESC/E(Z) complex; IDA:FlyBase.
 GO:0000790; C:nuclear chromatin; NAS:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0003677; F:DNA binding; NAS:UniProtKB.
 GO:0046976; F:histone methyltransferase activity (H3-K27 specific); IDA:FlyBase.
 GO:0046974; F:histone methyltransferase activity (H3-K9 specific); IDA:FlyBase.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0007411; P:axon guidance; IMP:FlyBase.
 GO:0006723; P:cuticle hydrocarbon biosynthetic process; IMP:FlyBase.
 GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
 GO:0016458; P:gene silencing; NAS:UniProtKB.
 GO:0007517; P:muscle organ development; IMP:FlyBase.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0035186; P:syncytial blastoderm mitotic cell cycle; IMP:FlyBase.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR026489; CXC_dom.
 IPR001005; SANT/Myb.
 IPR001214; SET_dom. 
Pfam
 PF00856; SET 
SMART
 SM00717; SANT
 SM00317; SET 
PROSITE
 PS51633; CXC
 PS51293; SANT
 PS50280; SET 
PRINTS