UniProt Accession

 CXA1_HUMAN; P17302; B2R5U9; Q6FHU1; Q9Y5I8 

Genbank Protein ID

 X52947; M65188; AF151980; CR541660; AK312324; AL139098; CH471051; BC026329 

Genbank Nucleotide ID

 CAA37122.1; AAA52131.1; AAD37802.2; CAG46461.1; BAG35246.1; CAI20002.1; EAW48178.1; AAH26329.1 

Protein Name

 Gap junction alpha-1 protein 

Protein Synonyms/Alias

 Connexin-43; Cx43; Gap junction 43 kDa heart protein 

Gene Name

 GJA1 

Gene Synonyms/Alias

 GJAL 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
9	GDWSALGKLLDKVQA	ubiquitination	[1, 2]
114	NKKEEELKVAQTDGV	ubiquitination	[3]
128	VNVDMHLKQIEIKKF	ubiquitination	[3, 4, 5]
136	QIEIKKFKYGIEEHG	ubiquitination	[3, 5]
243	VKDRVKGKSDPYHAT	ubiquitination	[3, 4]
258	SGALSPAKDCGSQKY	ubiquitination	[3, 5]
264	AKDCGSQKYAYFNGC	ubiquitination	[3, 5]
303	SSCRNYNKQASEQNW	ubiquitination	[1, 2, 3, 4, 5]
345	PDDNQNSKKLAAGHE	ubiquitination	[3, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). 

Sequence Annotation

 MOD_RES 247 247 Phosphotyrosine (By similarity).
 MOD_RES 255 255 Phosphoserine.
 MOD_RES 262 262 Phosphoserine.
 MOD_RES 271 271 S-nitrosocysteine (By similarity).
 MOD_RES 306 306 Phosphoserine.
 MOD_RES 313 313 Phosphotyrosine (By similarity).
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 325 325 Phosphoserine; by CK1.
 MOD_RES 326 326 Phosphothreonine (By similarity).
 MOD_RES 328 328 Phosphoserine; by CK1.
 MOD_RES 330 330 Phosphoserine; by CK1.
 MOD_RES 344 344 Phosphoserine.
 MOD_RES 365 365 Phosphoserine (By similarity).
 MOD_RES 368 368 Phosphoserine; by PKC/PRKCG (By
 MOD_RES 369 369 Phosphoserine (By similarity).
 MOD_RES 373 373 Phosphoserine (By similarity).
 DISULFID 54 192
 DISULFID 187 198
 CROSSLNK 144 144 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 237 237 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Cataract; Cell junction; Cell membrane; Complete proteome; Disease mutation; Disulfide bond; Gap junction; Isopeptide bond; Membrane; Phosphoprotein; Polymorphism; Reference proteome; S-nitrosylation; Transmembrane; Transmembrane helix; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 382 AA 

Protein Sequence

Gene Ontology

 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0005922; C:connexon complex; TAS:ProtInc.
 GO:0043292; C:contractile fiber; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005769; C:early endosome; IEA:Compara.
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0005916; C:fascia adherens; IEA:Compara.
 GO:0030660; C:Golgi-associated vesicle membrane; TAS:Reactome.
 GO:0005887; C:integral to plasma membrane; TAS:UniProtKB.
 GO:0014704; C:intercalated disc; IDA:BHF-UCL.
 GO:0005882; C:intermediate filament; IEA:Compara.
 GO:0016328; C:lateral plasma membrane; IEA:Compara.
 GO:0005764; C:lysosome; IEA:Compara.
 GO:0045121; C:membrane raft; ISS:BHF-UCL.
 GO:0005741; C:mitochondrial outer membrane; IEA:Compara.
 GO:0005771; C:multivesicular body; IEA:Compara.
 GO:0005243; F:gap junction channel activity; IDA:BHF-UCL.
 GO:0015075; F:ion transmembrane transporter activity; TAS:ProtInc.
 GO:0004871; F:signal transducer activity; IMP:UniProtKB.
 GO:0007512; P:adult heart development; IEA:Compara.
 GO:0006915; P:apoptotic process; IEA:Compara.
 GO:0015867; P:ATP transport; IEA:Compara.
 GO:0003294; P:atrial ventricular junction remodeling; IEA:Compara.
 GO:0048514; P:blood vessel morphogenesis; IEA:Compara.
 GO:0010643; P:cell communication by chemical coupling; IEA:Compara.
 GO:0010644; P:cell communication by electrical coupling; IDA:BHF-UCL.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0002070; P:epithelial cell maturation; IEA:Compara.
 GO:0016264; P:gap junction assembly; TAS:UniProtKB.
 GO:0007507; P:heart development; TAS:ProtInc.
 GO:0001947; P:heart looping; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0002088; P:lens development in camera-type eye; IEA:Compara.
 GO:0060174; P:limb bud formation; IEA:Compara.
 GO:0060156; P:milk ejection; IEA:Compara.
 GO:0006936; P:muscle contraction; TAS:ProtInc.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0001764; P:neuron migration; IEA:Compara.
 GO:0048812; P:neuron projection morphogenesis; IEA:Compara.
 GO:0001649; P:osteoblast differentiation; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0045732; P:positive regulation of protein catabolic process; IEA:Compara.
 GO:0045844; P:positive regulation of striated muscle tissue development; IEA:Compara.
 GO:0051259; P:protein oligomerization; IEA:Compara.
 GO:0086014; P:regulation of atrial cardiac muscle cell action potential; TAS:BHF-UCL.
 GO:0060371; P:regulation of atrial cardiac muscle cell membrane depolarization; IEA:Compara.
 GO:0030500; P:regulation of bone mineralization; IEA:Compara.
 GO:0046850; P:regulation of bone remodeling; IEA:Compara.
 GO:0051924; P:regulation of calcium ion transport; IEA:Compara.
 GO:2000810; P:regulation of tight junction assembly; IEA:Compara.
 GO:0060373; P:regulation of ventricular cardiac muscle cell membrane depolarization; IEA:Compara.
 GO:0060307; P:regulation of ventricular cardiac muscle cell membrane repolarization; IEA:Compara.
 GO:0043434; P:response to peptide hormone stimulus; IEA:Compara.
 GO:0009268; P:response to pH; IEA:Compara.
 GO:0043403; P:skeletal muscle tissue regeneration; IEA:Compara.
 GO:0010232; P:vascular transport; IEA:Compara. 

Interpro

 IPR000500; Connexin.
 IPR002261; Connexin43.
 IPR013124; Connexin43_C.
 IPR019570; Connexin_CCC.
 IPR017990; Connexin_CS.
 IPR013092; Connexin_N. 

Pfam

 PF00029; Connexin
 PF03508; Connexin43
 PF10582; Connexin_CCC 

SMART

 SM00037; CNX
 SM01089; Connexin_CCC 

PROSITE

 PS00407; CONNEXINS_1
 PS00408; CONNEXINS_2 

PRINTS

 PR00206; CONNEXIN.
 PR01132; CONNEXINA1.