CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005166
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NAD-dependent malic enzyme, mitochondrial 
Protein Synonyms/Alias
 NAD-ME; Malic enzyme 2 
Gene Name
 ME2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24RHLHIKEKGKPLMLNacetylation[1]
94GIQERNEKLFYRILQacetylation[2]
156NWPENHVKAVVVTDGacetylation[1, 2, 3, 4]
224FYMGLYQKRDRTQQYacetylation[2, 3, 4]
240DLIDEFMKAITDRYGacetylation[1, 2, 4]
272FLRKYREKYCTFNDDacetylation[2, 3]
346KKIWMFDKYGLLVKGacetylation[2]
346KKIWMFDKYGLLVKGubiquitination[5]
453GSPFGPVKLTDGRVFubiquitination[5]
538TEYLYANKMAFRYPEacetylation[4]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
 NP_BIND 165 173 NAD.
 NP_BIND 311 328 NAD.
 ACT_SITE 112 112 Proton donor.
 ACT_SITE 183 183 Proton acceptor.
 METAL 255 255 Divalent metal cation.
 METAL 256 256 Divalent metal cation.
 METAL 279 279 Divalent metal cation.
 BINDING 67 67 Allosteric activator.
 BINDING 91 91 Allosteric activator.
 BINDING 165 165 Substrate.
 BINDING 259 259 NAD.
 BINDING 421 421 Substrate.
 BINDING 466 466 Substrate.
 MOD_RES 156 156 N6-acetyllysine.
 MOD_RES 224 224 N6-acetyllysine.
 MOD_RES 240 240 N6-acetyllysine.
 MOD_RES 272 272 N6-acetyllysine.
 MOD_RES 346 346 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Allosteric enzyme; Alternative splicing; Complete proteome; Direct protein sequencing; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 584 AA 
Protein Sequence
MLSRLRVVST TCTLACRHLH IKEKGKPLML NPRTNKGMAF TLQERQMLGL QGLLPPKIET 60
QDIQALRFHR NLKKMTSPLE KYIYIMGIQE RNEKLFYRIL QDDIESLMPI VYTPTVGLAC 120
SQYGHIFRRP KGLFISISDR GHVRSIVDNW PENHVKAVVV TDGERILGLG DLGVYGMGIP 180
VGKLCLYTAC AGIRPDRCLP VCIDVGTDNI ALLKDPFYMG LYQKRDRTQQ YDDLIDEFMK 240
AITDRYGRNT LIQFEDFGNH NAFRFLRKYR EKYCTFNDDI QGTAAVALAG LLAAQKVISK 300
PISEHKILFL GAGEAALGIA NLIVMSMVEN GLSEQEAQKK IWMFDKYGLL VKGRKAKIDS 360
YQEPFTHSAP ESIPDTFEDA VNILKPSTII GVAGAGRLFT PDVIRAMASI NERPVIFALS 420
NPTAQAECTA EEAYTLTEGR CLFASGSPFG PVKLTDGRVF TPGQGNNVYI FPGVALAVIL 480
CNTRHISDSV FLEAAKALTS QLTDEELAQG RLYPPLANIQ EVSINIAIKV TEYLYANKMA 540
FRYPEPEDKA KYVKERTWRS EYDSLLPDVY EWPESASSPP VITE 584 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0009055; F:electron carrier activity; TAS:UniProtKB.
 GO:0004471; F:malate dehydrogenase (decarboxylating) activity; TAS:ProtInc.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IEA:InterPro. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.