CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016964
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Adipocyte enhancer-binding protein 1 
Protein Synonyms/Alias
 AE-binding protein 1; Aortic carboxypeptidase-like protein 
Gene Name
 AEBP1 
Gene Synonyms/Alias
 ACLP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
575KDMRQLMKVVNEECPubiquitination[1]
592TRTYSLGKSSRGLKIubiquitination[1]
598GKSSRGLKIYAMEISacetylation[2]
716LWGAEERKWVPYRVPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation (By similarity). May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I- kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness (By similarity). Can act as a transcriptional repressor (By similarity). 
Sequence Annotation
 DOMAIN 383 540 F5/8 type C.
 REGION 390 555 Required for DNA-binding and interaction
 REGION 421 624 Interaction with MAPK1 and MAPK3 (By
 REGION 555 985 Interaction with PTEN (By similarity).
 REGION 941 1158 Required for transcriptional repression
 REGION 1006 1158 Interaction with MAPK1 and MAPK3 (By
 CARBOHYD 528 528 N-linked (GlcNAc...).
 CARBOHYD 922 922 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Calmodulin-binding; Complete proteome; Cytoplasm; DNA-binding; Glycoprotein; Nucleus; Polymorphism; Reference proteome; Repressor; Secreted; Signal; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1158 AA 
Protein Sequence
MAAVRGAPLL SCLLALLALC PGGRPQTVLT DDEIEEFLEG FLSELEPEPR EDDVEAPPPP 60
EPTPRVRKAQ AGGKPGKRPG TAAEVPPEKT KDKGKKGKKD KGPKVPKESL EGSPRPPKKG 120
KEKPPKATKK PKEKPPKATK KPKEKPPKAT KKPKEKPPKA TKKPPSGKRP PILAPSETLE 180
WPLPPPPSPG PEELPQEGGA PLSNNWQNPG EETHVEAREH QPEPEEETEQ PTLDYNDQIE 240
REDYEDFEYI RRQKQPRPPP SRRRRPERVW PEPPEEKAPA PAPEERIEPP VKPLLPPLPP 300
DYGDGYVIPN YDDMDYYFGP PPPQKPDAER QTDEEKEELK KPKKEDSSPK EETDKWAVEK 360
GKDHKEPRKG EELEEEWTPT EKVKCPPIGM ESHRIEDNQI RASSMLRHGL GAQRGRLNMQ 420
TGATEDDYYD GAWCAEDDAR TQWIEVDTRR TTRFTGVITQ GRDSSIHDDF VTTFFVGFSN 480
DSQTWVMYTN GYEEMTFHGN VDKDTPVLSE LPEPVVARFI RIYPLTWNGS LCMRLEVLGC 540
SVAPVYSYYA QNEVVATDDL DFRHHSYKDM RQLMKVVNEE CPTITRTYSL GKSSRGLKIY 600
AMEISDNPGE HELGEPEFRY TAGIHGNEVL GRELLLLLMQ YLCREYRDGN PRVRSLVQDT 660
RIHLVPSLNP DGYEVAAQMG SEFGNWALGL WTEEGFDIFE DFPDLNSVLW GAEERKWVPY 720
RVPNNNLPIP ERYLSPDATV STEVRAIIAW MEKNPFVLGA NLNGGERLVS YPYDMARTPT 780
QEQLLAAAMA AARGEDEDEV SEAQETPDHA IFRWLAISFA SAHLTLTEPY RGGCQAQDYT 840
GGMGIVNGAK WNPRTGTIND FSYLHTNCLE LSFYLGCDKF PHESELPREW ENNKEALLTF 900
MEQVHRGIKG VVTDEQGIPI ANATISVSGI NHGVKTASGG DYWRILNPGE YRVTAHAEGY 960
TPSAKTCNVD YDIGATQCNF ILARSNWKRI REIMAMNGNR PIPHIDPSRP MTPQQRRLQQ 1020
RRLQHRLRLR AQMRLRRLNA TTTLGPHTVP PTLPPAPATT LSTTIEPWGL IPPTTAGWEE 1080
SETETYTEVV TEFGTEVEPE FGTKVEPEFE TQLEPEFETQ LEPEFEEEEE EEKEEEIATG 1140
QAFPFTTVET YTVNFGDF 1158 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005615; C:extracellular space; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004180; F:carboxypeptidase activity; TAS:ProtInc.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004181; F:metallocarboxypeptidase activity; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0003714; F:transcription corepressor activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007155; P:cell adhesion; IEA:InterPro.
 GO:0007517; P:muscle organ development; TAS:ProtInc.
 GO:0006508; P:proteolysis; IEA:InterPro.
 GO:0001501; P:skeletal system development; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR008969; CarboxyPept-like_regulatory.
 IPR014766; CarboxyPept_regulatory_dom.
 IPR000421; Coagulation_fac_5/8-C_type_dom.
 IPR008979; Galactose-bd-like.
 IPR000834; Peptidase_M14. 
Pfam
 PF00754; F5_F8_type_C
 PF00246; Peptidase_M14 
SMART
 SM00231; FA58C
 SM00631; Zn_pept 
PROSITE
 PS00132; CARBOXYPEPT_ZN_1
 PS01285; FA58C_1
 PS01286; FA58C_2
 PS50022; FA58C_3 
PRINTS
 PR00765; CRBOXYPTASEA.