CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009384
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 4-aminobutyrate aminotransferase, mitochondrial 
Protein Synonyms/Alias
 (S)-3-amino-2-methylpropionate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; GABA-T; L-AIBAT 
Gene Name
 Abat 
Gene Synonyms/Alias
 Gabat 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
60PRSKELMKQLNTIQNacetylation[1]
252IAPFPRLKYPLEEFTacetylation[1, 2, 3, 4]
252IAPFPRLKYPLEEFTsuccinylation[3]
279EVEDLIVKYRKKKRTacetylation[1, 2, 4, 5]
318KLRDIARKHGCAFLVacetylation[2, 6]
400AEVINIIKREDLLNNacetylation[2]
413NNVARVGKTLLTGLLacetylation[2, 3]
413NNVARVGKTLLTGLLsuccinylation[3]
452PDEAIRNKLILIARNacetylation[1, 2, 4, 7, 8]
470VLGGCGDKSIRFRPTacetylation[1, 2, 4]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [7] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [8] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599] 
Functional Description
 Catalyzes the conversion of gamma-aminobutyrate and L- beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine (By similarity). 
Sequence Annotation
 MOD_RES 318 318 N6-acetyllysine.
 MOD_RES 357 357 N6-(pyridoxal phosphate)lysine (By
 DISULFID 163 166 By similarity.
 DISULFID 321 321 Interchain (By similarity).  
Keyword
 Acetylation; Alternative splicing; Aminotransferase; Complete proteome; Direct protein sequencing; Disulfide bond; Mitochondrion; Neurotransmitter degradation; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 500 AA 
Protein Sequence
MAFLLITRRL ACSSQKNLHL FIPGSRYISQ AAAKVDIEFD YDGPLMKTEV PGPRSKELMK 60
QLNTIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI SSVPIGYNHP ALAKLVQQPQ 120
NASTFINRPA LGILPPENFV DKLQESLMSV APRGMSQLIT MACGSCSNEN AFKTIFMWYR 180
SKERGQRGFS KEELETCMVN QSPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS 240
FDWPIAPFPR LKYPLEEFTT DNQQEEARCL EEVEDLIVKY RKKKRTVAGI IVEPIQSEGG 300
DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA DVMTFSKKMM 360
TGGFFHKEEF RPSAPYRIFN TWLGDPSKNL LLAEVINIIK REDLLNNVAR VGKTLLTGLL 420
DLQAQYPQFI SRVRGRGTFC SFDTPDEAIR NKLILIARNK GVVLGGCGDK SIRFRPTLVF 480
RDHHAHLFLS IFSGILADFK 500 
Gene Ontology
 GO:0032144; C:4-aminobutyrate transaminase complex; ISS:UniProtKB.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0047298; F:(S)-3-amino-2-methylpropionate transaminase activity; IEA:EC.
 GO:0003867; F:4-aminobutyrate transaminase activity; IEA:EC.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0030170; F:pyridoxal phosphate binding; ISS:UniProtKB.
 GO:0032145; F:succinate-semialdehyde dehydrogenase binding; ISS:UniProtKB.
 GO:0048148; P:behavioral response to cocaine; IMP:UniProtKB.
 GO:0007620; P:copulation; IEA:Compara.
 GO:0009448; P:gamma-aminobutyric acid metabolic process; IEA:InterPro.
 GO:0007626; P:locomotory behavior; IEA:Compara.
 GO:0045776; P:negative regulation of blood pressure; IEA:Compara.
 GO:0042135; P:neurotransmitter catabolic process; IEA:UniProtKB-KW.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0010039; P:response to iron ion; IEA:Compara.
 GO:0035094; P:response to nicotine; IEA:Compara. 
Interpro
 IPR004631; 4NH2But_aminotransferase_euk.
 IPR005814; Aminotrans_3.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 
Pfam
 PF00202; Aminotran_3 
SMART
  
PROSITE
 PS00600; AA_TRANSFER_CLASS_3 
PRINTS