CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023586
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase 
Protein Synonyms/Alias
 UDP-GlcNAc-2-epimerase/ManAc kinase; UDP-N-acetylglucosamine 2-epimerase (hydrolyzing); UDP-GlcNAc-2-epimerase; Uridine diphosphate-N-acetylglucosamine-2-epimerase; N-acetylmannosamine kinase; ManAc kinase 
Gene Name
 GNE 
Gene Synonyms/Alias
 GLCNE 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
55CNRADYSKLAPIMFGubiquitination[1]
226KLLSAKNKDYMSIIRubiquitination[1, 2]
241MWLGDDVKSKDYIVAubiquitination[1]
243LGDDVKSKDYIVALQubiquitination[1]
290PNIDAGSKEMVRVMRubiquitination[1]
299MVRVMRKKGIEHHPNubiquitination[1]
373RDADTQDKILQALHLubiquitination[1]
384ALHLQFGKQYPCSKIubiquitination[1]
390GKQYPCSKIYGDGNAubiquitination[1]
406PRILKFLKSIDLQEPubiquitination[1]
416DLQEPLQKKFCFPPVubiquitination[1]
417LQEPLQKKFCFPPVKubiquitination[1]
521GIVLHSTKLIQEWNSubiquitination[1]
668AAKLGNAKAQSILRTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Regulates and initiates biosynthesis of N- acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. 
Sequence Annotation
 NP_BIND 411 420 ATP.
 NP_BIND 543 552 ATP.
 REGION 1 ? UDP-N-acetylglucosamine 2-epimerase.
 REGION 406 722 N-acetylmannosamine kinase.
 ACT_SITE 517 517
 METAL 569 569 Zinc.
 METAL 579 579 Zinc.
 METAL 581 581 Zinc.
 METAL 586 586 Zinc.
 BINDING 477 477 Substrate.
 BINDING 489 489 Substrate.
 BINDING 517 517 Substrate.
 BINDING 566 566 Substrate.
 BINDING 569 569 Substrate.
 BINDING 588 588 Substrate.  
Keyword
 3D-structure; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Disease mutation; Hydrolase; Kinase; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 722 AA 
Protein Sequence
METYGYLQRE SCFQGPHELY FKNLSKRNKQ IMEKNGNNRK LRVCVATCNR ADYSKLAPIM 60
FGIKTEPEFF ELDVVVLGSH LIDDYGNTYR MIEQDDFDIN TRLHTIVRGE DEAAMVESVG 120
LALVKLPDVL NRLKPDIMIV HGDRFDALAL ATSAALMNIR ILHIEGGEVS GTIDDSIRHA 180
ITKLAHYHVC CTRSAEQHLI SMCEDHDRIL LAGCPSYDKL LSAKNKDYMS IIRMWLGDDV 240
KSKDYIVALQ HPVTTDIKHS IKMFELTLDA LISFNKRTLV LFPNIDAGSK EMVRVMRKKG 300
IEHHPNFRAV KHVPFDQFIQ LVAHAGCMIG NSSCGVREVG AFGTPVINLG TRQIGRETGE 360
NVLHVRDADT QDKILQALHL QFGKQYPCSK IYGDGNAVPR ILKFLKSIDL QEPLQKKFCF 420
PPVKENISQD IDHILETLSA LAVDLGGTNL RVAIVSMKGE IVKKYTQFNP KTYEERINLI 480
LQMCVEAAAE AVKLNCRILG VGISTGGRVN PREGIVLHST KLIQEWNSVD LRTPLSDTLH 540
LPVWVDNDGN CAALAERKFG QGKGLENFVT LITGTGIGGG IIHQHELIHG SSFCAAELGH 600
LVVSLDGPDC SCGSHGCIEA YASGMALQRE AKKLHDEDLL LVEGMSVPKD EAVGALHLIQ 660
AAKLGNAKAQ SILRTAGTAL GLGVVNILHT MNPSLVILSG VLASHYIHIV KDVIRQQALS 720
SVQDVDVVVS DLVDPALLGA ASMVLDYTTR RIY 753 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009384; F:N-acylmannosamine kinase activity; TAS:ProtInc.
 GO:0008761; F:UDP-N-acetylglucosamine 2-epimerase activity; TAS:ProtInc.
 GO:0007155; P:cell adhesion; TAS:ProtInc.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
 GO:0006045; P:N-acetylglucosamine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0046380; P:N-acetylneuraminate biosynthetic process; IEA:InterPro.
 GO:0006054; P:N-acetylneuraminate metabolic process; TAS:ProtInc.
 GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IEA:InterPro. 
Interpro
 IPR001312; Hexokinase.
 IPR000600; ROK.
 IPR020004; UDP-GlcNAc_Epase.
 IPR003331; UDP_GlcNAc_Epimerase_2. 
Pfam
 PF02350; Epimerase_2
 PF00480; ROK 
SMART
  
PROSITE
 PS01125; ROK 
PRINTS
 PR00475; HEXOKINASE.