UniProt Accession

 NB5R3_BOVIN; P07514; A6H7G0 

Genbank Protein ID

 BC146232; M83104 

Genbank Nucleotide ID

 AAI46233.1; AAA30483.1 

Protein Name

 NADH-cytochrome b5 reductase 3 

Protein Synonyms/Alias

 B5R; Cytochrome b5 reductase; Diaphorase-1; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form 

Gene Name

 CYB5R3 

Gene Synonyms/Alias

 DIA1 

Created Date

 July 27, 2013 

Organism

 Bos taurus (Bovine) 

NCBI Taxa ID

 9913 

Lysine Modification

Position	Peptide	Type	References
111	GFVDLVIKVYFKDTH	acetylation	[1]

Reference

 [1] NADH binding to cytochrome b5 reductase blocks the acetylation of lysine 110.
 Hackett CS, Novoa WB, Kensil CR, Strittmatter P.
 J Biol Chem. 1988 Jun 5;263(16):7539-43. [PMID: 3131323] 

Functional Description

 Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. 

Sequence Annotation

 DOMAIN 40 152 FAD-binding FR-type.
 NP_BIND 132 147 FAD (By similarity).
 NP_BIND 171 206 FAD (By similarity).
 MOD_RES 42 42 N6-acetyllysine (By similarity).
 MOD_RES 43 43 Phosphotyrosine (By similarity).
 MOD_RES 120 120 N6-acetyllysine (By similarity).
 MOD_RES 130 130 Phosphotyrosine (By similarity).
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 Acetylation; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Lipoprotein; Membrane; Mitochondrion; Mitochondrion outer membrane; Myristate; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 301 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IEA:EC.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR 

PRINTS

 PR00406; CYTB5RDTASE.
 PR00371; FPNCR.