CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002731
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoglycerate kinase 2 
Protein Synonyms/Alias
 Phosphoglycerate kinase, testis specific 
Gene Name
 Pgk2 
Gene Synonyms/Alias
 Pgk-2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
11SAKLTLDKVDLKGKRubiquitination[1]
30VDFNVPMKNNQITNNacetylation[2, 3, 4]
30VDFNVPMKNNQITNNsuccinylation[4]
30VDFNVPMKNNQITNNubiquitination[1]
131FHVEEEGKGKDSSGKacetylation[2, 4, 5]
156AFQASLSKLGDVYVNacetylation[3, 6]
156AFQASLSKLGDVYVNubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
 NP_BIND 373 376 ATP (By similarity).
 REGION 24 26 Substrate binding (By similarity).
 REGION 63 66 Substrate binding (By similarity).
 BINDING 39 39 Substrate (By similarity).
 BINDING 123 123 Substrate (By similarity).
 BINDING 171 171 Substrate (By similarity).
 BINDING 220 220 ATP (By similarity).
 BINDING 313 313 ATP; via carbonyl oxygen (By similarity).
 BINDING 344 344 ATP (By similarity).  
Keyword
 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 417 AA 
Protein Sequence
MALSAKLTLD KVDLKGKRVI MRVDFNVPMK NNQITNNQRI KAAIPSIKHC LDNGAKSVVL 60
MSHLGRPDGI PMPDKYSLEP VADELKSLLN KDVIFLKDCV GPEVEQACAN PDNGSIILLE 120
NLRFHVEEEG KGKDSSGKKI SADPAKVEAF QASLSKLGDV YVNDAFGTAH RAHSSTVGVN 180
LPQKASGFLM KKELDYFSKA LEKPERPFLA ILGGAKVKDK IQLIKNMLDK VNFMIIGGGM 240
AYTFLKELKN MQIGASLFDE EGATIVKEIM EKAEKNGVKI VFPVDFVTGD KFDENAKVGQ 300
ATIESGIPSG WMGLDCGPES IKINAQIVAQ AKLIVWNGPI GVFEWDAFAK GTKALMDEVV 360
KATSNGCVTI IGGGDTATCC AKWGTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM 417 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0004618; F:phosphoglycerate kinase activity; ISS:UniProtKB.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway. 
Interpro
 IPR001576; Phosphoglycerate_kinase.
 IPR015901; Phosphoglycerate_kinase_C.
 IPR015911; Phosphoglycerate_kinase_CS.
 IPR015824; Phosphoglycerate_kinase_N. 
Pfam
 PF00162; PGK 
SMART
  
PROSITE
 PS00111; PGLYCERATE_KINASE 
PRINTS
 PR00477; PHGLYCKINASE.